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ZFIN ID: ZDB-FIG-071228-18
Veldman et al., 2007 - Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration. Developmental Biology   312(2):596-612 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Adult

Fig. 2 In situ hybridization verification of microarray results and comparison with expression in the developing retina (part I). mRNA for Tubb5 (A–C), Pak1 (D–F), RhoGDIα (G–I), Rad (J–L), Rac1 (M–O), Creb3l3 (P–R), Crem/Icer (S–U), and bHLHB2 (V–X) was detected by in situ hybridization in uninjured control, 3-day post-optic nerve crush and 48 hpf retinal sections. Note that mRNA levels are elevated in the ganglion cell layer (GCL) of Tubb5, Pak1, RhoGDIα, Rad, Rac1, Creb3l3, and Crem/Icer probed sections following nerve crush injury. bHLHB2 is decreased in the GCL following nerve crush injury. Developmentally, Tubb5, Pak1, RhoGDIα, and Creb3l3 are all expressed in the GCL at 48 hpf (arrows in panels C, F, I, and R). Rad and Crem/Icer are not expressed in the retina at 48 hpf (L and U). Rac1 and bHLHB2 are expressed weakly but uniformly in the retina at 48 hpf (O and X). Sense probes for all genes tested gave no specific signal (data not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arhgdia WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
bhlhe40 WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retina ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
creb3l3l WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
cremb WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec Not Detected retina ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
pak1 WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
rac1a WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retina ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
rrad WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec Not Detected retina ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
tubb5 WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 312(2), Veldman, M.B., Bemben, M.A., Thompson, R.C., and Goldman, D., Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration, 596-612, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.