FIGURE

Fig. 1

ID

ZDB-FIG-071228-17

Publication

Veldman et al., 2007 - Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Laser capture microdissection (LCM) of RGCs and linear amplification of RNA isolated from these cells. (A) Images of a cresyl violet stained retinal section before RGC capture (top), with area to be captured highlighted in red (middle) and after RGC capture (bottom) LCM. (B) Schematic lateral view of the fish eye with lines representing the distribution of sections taken for RGC harvesting. Dorsal (D), ventral (V), rostral (R), and caudal (C). (C) Quantitative RT-PCR results from 1st strand cDNA and amplified cDNA (a-cDNA) showing that α1 tubulin was induced as expected in optic nerve-lesioned RGCs and that amplification was linear and accurately reflected α1 tubulin induction. These samples were used to prepare probes for the microarray hybridization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 312(2), Veldman, M.B., Bemben, M.A., Thompson, R.C., and Goldman, D., Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration, 596-612, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.