ZFIN ID: ZDB-FIG-071228-19
Veldman et al., 2007 - Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration. Developmental Biology   312(2):596-612 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Stage Range: Long-pec to Adult

Fig. 3 In situ hybridization verification of microarray results and comparison with expression in the developing retina (part II). mRNA for SOCS3a (A–C), SOCS3b (D–F), Sox11a (G–I), Sox11b (J–L), KLF6a (M–O), and KLF7a (P–R) was detected by in situ hybridization in uninjured control, 3-day post-optic nerve crush and 48 hpf retinal sections. Expression of all six genes is elevated in the ganglion cell layer (GCL) following optic nerve crush injury as compared to control. Developmentally, Sox11a, Sox11b, and KLF7a are expressed in the GCL at 48 hpf while SOCS3a, SOCS3b, and KLF6a are not (arrows in panels C, F, I, L, O, and R). Sense probes for all genes tested gave no specific signal (data not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
klf6a WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec Not Detected retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
klf7b WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
socs3a WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec Not Detected retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
socs3b WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec Not Detected retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
sox11a WT control Adult retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
sox11b WT control Adult Not Detected retinal ganglion cell layer ISH
WT standard conditions Long-pec retinal ganglion cell layer ISH
WT physical alteration: anatomical structure Adult retinal ganglion cell layer ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 312(2), Veldman, M.B., Bemben, M.A., Thompson, R.C., and Goldman, D., Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration, 596-612, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.