ZFIN ID: ZDB-FIG-070307-73
Hans et al., 2007 - Fgf-dependent otic induction requires competence provided by Foxi1 and Dlx3b. BMC Developmental Biology   7(1):5 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: 1-4 somites

Fig. 5 Ubiquitous fgf8 expression at late gastrulation stages leads to ectopic otic induction within the preotic field. (A, G) Ectopic fgf8 expression can be detected throughout the embryo after a 30 minute heat shock in transgenic hsp:fgf8 embryos in comparison to non-transgenic embryos. (B, C, H, I) Within 2 hours, expression of the two Fgf reporter genes, erm and pea3, is upregulated in cells of the transgenic embryos whereas non-transgenic siblings are unaffected. (D-F, J-L) After misexpression of fgf8 at late gastrulation stages, expression of foxi1 is reduced, whereas the preotic expression domains of pax8 and pax2a are enlarged. Dorsal views of 2–5-somite stage embryos with anterior towards the top. mhb, midbrain-hindbrain border; po, preotic region; p, pronephros. Scale bar: 100 μm for A-C, G-I; 40 μm for D-F, J-L.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 WT standard conditions 1-4 somites mesoderm ISH
1-4 somites neural keel ISH
1-4 somites segmental plate ISH
bmp7aty68a/ty68a heat shock 1-4 somites whole organism ISH
etv5b WT standard conditions 1-4 somites mesoderm ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites segmental plate ISH
bmp7aty68a/ty68a heat shock 1-4 somites whole organism ISH
fgf8a WT standard conditions 1-4 somites mesoderm ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
bmp7aty68a/ty68a heat shock 1-4 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.