ZFIN ID: ZDB-FIG-070307-75
Hans et al., 2007 - Fgf-dependent otic induction requires competence provided by Foxi1 and Dlx3b. BMC Developmental Biology   7(1):5 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7 Ectopic otic induction after fgf8 expression at late gastrulation stages requires both, Foxi1 and Dlx3b. (A-D) Inactivation of Dlx3b or Foxi1 in wild-type embryos by morpholino injection (MO) leads to a reduction of ear size in comparison to wild-type embryos, and combined loss of Dlx3b and Foxi1 results in loss of all indications of otic specification. (E-H) Ear size reduction by depletion of Dlx3b or Foxi1 but not combined loss of Dlx3b and Foxi1, can be partially rescued in transgenic hsp:fgf8 embryos heat shocked at late gastrulation stages. Lateral views of otic vesicles hybridized with starmaker at 24h with anterior to the left and dorsal towards the top. Scale bar: 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
stm WT standard conditions Prim-5 otic vesicle ISH
WT + MO1-dlx3b standard conditions Prim-5 otic vesicle ISH
WT + MO1-dlx3b + MO2-foxi1 standard conditions Prim-5 Not Detected otic vesicle ISH
WT + MO2-foxi1 standard conditions Prim-5 otic vesicle ISH
b1193Tg(AB) heat shock Prim-5 otic vesicle ISH
b1193Tg + MO1-dlx3b(AB) heat shock Prim-5 otic vesicle ISH
b1193Tg + MO1-dlx3b + MO2-foxi1(AB) heat shock Prim-5 Not Detected otic vesicle ISH
b1193Tg + MO2-foxi1(AB) heat shock Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dlx3b standard conditions Prim-5 otic vesicle decreased size, abnormal
WT + MO1-dlx3b + MO2-foxi1 standard conditions Prim-5 otic vesicle aplastic, abnormal
WT + MO2-foxi1 standard conditions Prim-5 otic vesicle decreased size, abnormal
b1193Tg + MO1-dlx3b(AB) heat shock Prim-5 otic vesicle decreased size, abnormal
b1193Tg + MO1-dlx3b + MO2-foxi1(AB) heat shock Prim-5 otic vesicle aplastic, abnormal
b1193Tg + MO2-foxi1(AB) heat shock Prim-5 otic vesicle decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.