ZFIN ID: ZDB-FIG-070307-77
Hans et al., 2007 - Fgf-dependent otic induction requires competence provided by Foxi1 and Dlx3b. BMC Developmental Biology   7(1):5 Full text @ BMC Dev. Biol.
Anatomical Terms:
Stage: 1-4 somites

Fig. S1 Heat shock at the end of gastrulation produces strong and ubiquitous fgf8 expression in transgenic animals. (A-C, G-K) Following a 30 minute heat shock, strong and ubiquitous expression of fgf8 can be observed in the transgenic embryos (G-K) up to 1.5 hours after heat shock, masking the endogenous fgf8 expression domains that are observed in the wild-type embryos (A-C). (D, I) Ectopic fgf8 mRNA is gradually lost and the endogenous fgf8 expression domains emerge at 2 hours after heat shock. (D, I) At 2.5 hours after heat shock only scattered cells show ectopic fgf8 expression in transgenic embryos. Dorsal views of 2–5-somite stage embryos with anterior towards the top. h, hours after heat shock; mhb, midbrain-hindbrain border; tb, tail bud. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fgf8a WT standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites tail bud ISH
b1193Tg heat shock 1-4 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.