ZFIN ID: ZDB-FIG-070307-76
Hans et al., 2007 - Fgf-dependent otic induction requires competence provided by Foxi1 and Dlx3b. BMC Developmental Biology   7(1):5 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: 1-4 somites

Fig. 8 Ectopic foxi1 expression after treatment with retinoic acid (RA) results in ectopic Fgf-dependent otic induction. (A, B, E, F) In comparison to wild-type control embryos treated with DMSO, embryos treated with 20nM RA show ectopic pax8 and foxi1 expression surrounding the anterior neural plate border without affecting the neural expression of otx2. (C, G) In fgf3, fgf8 double mutants, pax8 is completely abolished in the control embryos, whereas RA-treated double mutant embryos show weak anterior expression of pax8. (D, H) In foxi1 mutants treated with DMSO, pax8 expression can not be detected in the preotic region; but in foxi1 mutants treated with RA, residual anterior pax8 expression is present. Dorsal views of 1–3-somite stage embryos with anterior towards the top. Scale bar: 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxi1 WT control 1-4 somites otic placode ISH
otx2b WT control 1-4 somites neural keel ISH
WT chemical treatment: all-trans-retinoic acid 1-4 somites neural keel ISH
fgf3t24149/t24149; fgf8ati282a/ti282a control 1-4 somites neural keel ISH
fgf3t24149/t24149; fgf8ati282a/ti282a chemical treatment: all-trans-retinoic acid 1-4 somites neural keel ISH
foxi1em1/em1 control 1-4 somites neural keel ISH
foxi1em1/em1 chemical treatment: all-trans-retinoic acid 1-4 somites neural keel ISH
pax8 WT control 1-4 somites otic placode ISH
fgf3t24149/t24149; fgf8ati282a/ti282a control 1-4 somites Not Detected otic placode ISH
foxi1em1/em1 control 1-4 somites Not Detected otic placode ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.