ZFIN ID: ZDB-FIG-070307-74
Hans et al., 2007 - Fgf-dependent otic induction requires competence provided by Foxi1 and Dlx3b. BMC Developmental Biology   7(1):5 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 6 Ectopic otic induction after fgf8 expression at late gastrulation stages leads to formation of larger but correctly patterned placodes and vesicles. (A, E) Otic placodes labeled by cldna expression are increased in size in transgenic hsp:fgf8 embryos in comparison to non-transgenic embryos following a heat shock at late gastrulation stages. (B-D, F-H) The enlarged otic vesicles of transgenic embryos show no apparent patterning defects in comparison to non-transgenic siblings assessed by morphology and marker gene expression, including otx1 and pax2a. (A, E) Dorsal views of 12-somite stage embryos with anterior towards the top; (B-D, F-H) lateral views of otic vesicles at 24h with anterior to the left and dorsal towards the top. o, otolith. Scale bar: 50 μm for A, E; 30 μm for B-D, F-H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cldna WT standard conditions 10-13 somites otic placode ISH
bmp7aty68a/ty68a heat shock 10-13 somites otic placode ISH
otx1 WT standard conditions Prim-5 otic vesicle ISH
bmp7aty68a/ty68a heat shock Prim-5 otic vesicle ISH
pax2a WT standard conditions Prim-5 otic vesicle ISH
bmp7aty68a/ty68a heat shock Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1193Tg heat shock Prim-5 otic vesicle increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.