FIGURE

Fig. 1

ID

ZDB-FIG-061215-14

Publication

Covassin et al., 2006 - Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish

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Fig. 1

Enrichment of GFP⁺ cells from Tg(fli1:egfp)^y1 zebrafish embryos. (A-D) Diagnostic fluorescence activated cell sorting of dissociated Tg(fli1:egfp)^y1 embryos. (A) Cells from Tg(fli1:egfp)^y1 embryos. (B) Cells from non-transgenic wild type embryos. (A, B) P3 and P4 demarcate cells sorted as GFP^- and GFP⁺, respectively. (C) GFP^- cells following FACS isolation. (D) GFP⁺ cells following FACS isolation. (E) Relative expression of indicated genes in GFP⁺ (black bars) and GFP^- (gray bars) determined by quantitative RT-PCR. Relative expression levels were determined by normalization to ef1a.

Expression Data

Genes:	dlb dlx2a fli1 flt4 gapdh kdrl ptprn2 tbx16l (all 8)
Fish:	WT
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 299(2), Covassin, L., Amigo, J.D., Suzuki, K., Teplyuk, V., Straubhaar, J., and Lawson, N.D., Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish, 551-562, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.