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Fig. 1 Enrichment of GFP+ cells from Tg(fli1:egfp)y1 zebrafish embryos. (A-D) Diagnostic fluorescence activated cell sorting of dissociated Tg(fli1:egfp)y1 embryos. (A) Cells from Tg(fli1:egfp)y1 embryos. (B) Cells from non-transgenic wild type embryos. (A, B) P3 and P4 demarcate cells sorted as GFP- and GFP+, respectively. (C) GFP- cells following FACS isolation. (D) GFP+ cells following FACS isolation. (E) Relative expression of indicated genes in GFP+ (black bars) and GFP- (gray bars) determined by quantitative RT-PCR. Relative expression levels were determined by normalization to ef1a.
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Reprinted from Developmental Biology, 299(2), Covassin, L., Amigo, J.D., Suzuki, K., Teplyuk, V., Straubhaar, J., and Lawson, N.D., Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish, 551-562, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.