Okuda et al., 2006 - Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution. Developmental dynamics : an official publication of the American Association of Anatomists   235(3):811-825 Full text @ Dev. Dyn.

Fig. 3 Temporal expression profiles of the zebrafish group B1 sox genes determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA isolates were prepared from embryos at six different stages (32 cells, 1,000 cells, 30% epiboly, tail bud, 24 hr, and 48 hr) and subjected to RT-PCR analysis. Both β-actin and ef1 were used as controls. No PCR products were detected when reverse transcriptase was omitted from the reaction (data not shown).

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Stage Range: 32-cell to Long-pec

Fig. 4 The expression of zebrafish group B1 sox genes from the shield to the epiboly stages of embryonic development, visualized by whole-mount in situ hybridization. A: Lateral views of shield stage embryos with the dorsal side toward the right. B: Lateral views of 75% epiboly stage embryos with the dorsal side toward the right (a-d) and dorsal views with animal toward the top (e-h). Double labeling shows sox expression in blue, and no tail expression in red (A,B). C: Chicken Sox2 expression at early HH stage 5 (a) and Sox3 expression at late HH stage 5 (b).

Fig. 5 The expression of zebrafish group B1 sox genes from the tail bud to three-somite stages of embryonic development. A: Dorsal views of tail bud stage embryos with the anterior toward the top. Double labeling shows sox expression in blue and pax2a (f-j) and anf (k-o) expression in red. B: Dorsal views of three-somite stage embryos with the anterior toward the top(a-l); lateral views of three-somite stage embryos with the dorsal side toward the right(m-r). krox20 expression is shown in red (g-l). C: Chicken Sox expression at HH stage 8 (4 somites).

Fig. 6 The expression of zebrafish group B1 sox genes from the 12-somite stage to the 25 hr postfertilization (hpf) stage. A: Dorsal (a-f) and lateral (g-l) views of 12-somite stage zebrafish embryos. B: Dorsal (a-f) and lateral (g-l) views of 21-somite stage embryos. C: Dorsal (a-f) and lateral (g-l) views of 25 hpf embryos. cb, cerebellum; fmb, forebrain-midbrain boundary; le, lens; mhb, midbrain-hindbrain boundary; olp, olfactory placode; otp, otic placode; ov, optic vesicle; re, retina. D: Chicken Sox expression at Hamburger and Hamilton stages 11 (13 somites; a-c) and 14 (22 somites; d-f).

Unillustrated author statements

EXPRESSION / LABELING:
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Stage: Shield
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.