ZFIN ID: ZDB-PERS-960805-60
Blader, Patrick
Email: patrick.blader@univ-tlse3.fr
URL: http://www-cbd.ups-tlse.fr/spip.php?article67
Affiliation: Blader Lab
Address: Centre de Biologie du Développement (UMR 5547) Centre National de Recherche Scientifique Université Paul Sabatier 118, Rte de Narbonne Toulouse, 31062 France
Country: France
Phone: 33-05-61-55-67-34
Fax: 33-05-61-55-65-07
Orcid ID:

The vertebrate central nervous system (CNS) is capable of highly complex functions, which are achieved in part thanks to the wide variety of neurons from which it is constructed. Thus, the development of the CNS requires that the appropriate neurons be born at the correct time and place and in the correct numbers. We are aiming to understand how these processes are controlled during development. Our work concerns three axes of research, one primarily historic and two more recent, all of which deal with various aspects of neurogenesis using the zebrafish as a model.

1) Previously, we have studied regulatory element required for the expression of the zebrafish proneural gene neurogenin1 (neurog1). We are now addressing how this bHLH transcription factor regulates its targets.More recently, we have shown that, neurog1 and a second zebrafish Atonal genes neurod4, are redundantly required for development of neurons in the olfactory epithelium.

2) The habenulae are bilateral nuclei in the epithalamus that display left-right (L/R) asymmetries of molecular markers and neural projections. Studies have shown that handedness of this asymmetry is biased by unilateral Nodal signalling. We have shown a L/R asymmetry in the pool of neurog1+ habenular progenitors and that this asymmetry is compromised in the absence of Nodal signalling. We are presently addressing the question of how Nodal signalling induces a left sided bias in habenular neurogenesis.

3) The zebrafish epiphysis is a small, light-sensitive vesicle in the epithalamus containing only two neural subtype, projection neurons or photoreceptors, and as such provides a simple system for understanding how different neural subtypes are specified. We have identified the Notch pathway as an actor in making the choice between these two cell fates. Our results suggest that at least two distinct signals are required for epiphysial neural fate specification: one as yet unknown signal for the induction of the photoreceptor fate and the other, involving Notch, for the inhibition of projection neuron traits. Via a small candidate screen, we recently identified BMP signalling as both necessary and sufficient to promote the photoreceptor fate. We have also demonstrate that crosstalk between BMP and Notch signalling is required for the inhibition of a projection neuron fate in future photoreceptors.

Hoijman, E., Fargas, L., Blader, P., Alsina, B. (2017) Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification. eLIFE. 6
Delfino-Machín, M., Madelaine, R., Busolin, G., Nikaido, M., Colanesi, S., Camargo-Sosa, K., Law, E.W., Toppo, S., Blader, P., Tiso, N., Kelsh, R.N. (2017) Sox10 contributes to the balance of fate choice in dorsal root ganglion progenitors. PLoS One. 12:e0172947
Halluin, C., Madelaine, R., Naye, F., Peers, B., Roussigné, M., Blader, P. (2016) Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade. PLoS One. 11:e0158210
Aguillon, R., Blader, P., Batut, J. (2016) Patterning, morphogenesis, and neurogenesis of zebrafish cranial sensory placodes. Methods in cell biology. 134:33-67
Hüsken, U., Stickney, H.L., Gestri, G., Bianco, I.H., Faro, A., Young, R.M., Roussigne, M., Hawkins, T.A., Beretta, C.A., Brinkmann, I., Paolini, A., Jacinto, R., Albadri, S., Dreosti, E., Tsalavouta, M., Schwarz, Q., Cavodeassi, F., Barth, A.K., Wen, L., Zhang, B., Blader, P., Yaksi, E., Poggi, L., Zigman, M., Lin, S., Wilson, S.W., Carl, M. (2014) Tcf7l2 Is Required for Left-Right Asymmetric Differentiation of Habenular Neurons. Current biology : CB. 24(19):2217-27
Garric, L., Ronsin, B., Roussigné, M., Booton, S., Gamse, J.T., Dufourcq, P., Blader, P. (2014) Pitx2c ensures habenular asymmetry by restricting parapineal cell number. Development (Cambridge, England). 141:1572-9
Kishimoto, N., Asakawa, K., Madelaine, R., Blader, P., Kawakami, K., and Sawamoto, K. (2013) Interhemispheric asymmetry of olfactory input-dependent neuronal specification in the adult brain. Nature Neuroscience. 16(7):884-8
Roussigne, M., Blader, P., and Wilson, S.W. (2012) Breaking symmetry: the zebrafish as a model for understanding left-right asymmetry in the developing brain. Developmental Neurobiology. 72(3):269-281
Madelaine, R., Garric, L., and Blader, P. (2011) Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England). 138(21):4753-4762
Quillien, A., Blanco-Sanchez, B., Halluin, C., Moore, J.C., Lawson, N.D., Blader, P., and Cau, E. (2011) BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development (Cambridge, England). 138(11):2293-2302
Madelaine, R., and Blader, P. (2011) A cluster of non-redundant Ngn1 binding sites is required for regulation of deltaA expression in zebrafish. Developmental Biology. 350(1):198-207
Chapouton, P., Skupien, P., Hesl, B., Coolen, M., Moore, J.C., Madelaine, R., Kremmer, E., Faus-Kessler, T., Blader, P., Lawson, N.D., and Bally-Cuif, L. (2010) Notch activity levels control the balance between quiescence and recruitment of adult neural stem cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(23):7961-7974
Cau, E., and Blader, P. (2009) Notch activity in the nervous system: to switch or not switch?. Neural Development. 4:36
Roussigné, M., Bianco, I.H., Wilson, S.W., and Blader, P. (2009) Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei. Development (Cambridge, England). 136(9):1549-1557
Cau, E., Quillien, A., and Blader, P. (2008) Notch resolves mixed neural identities in the zebrafish epiphysis. Development (Cambridge, England). 135(14):2391-2401
Langevin, L.M., Mattar, P., Scardigli, R., Roussigne, M., Logan, C., Blader, P., and Schuurmans, C. (2007) Validating in utero electroporation for the rapid analysis of gene regulatory elements in the murine telencephalon. Developmental dynamics : an official publication of the American Association of Anatomists. 236(5):1273-1286
Carney, T.J., Dutton, K.A., Greenhill, E., Delfino-Machin, M., Dufourcq, P., Blader, P., and Kelsh, R.N. (2006) A direct role for Sox10 in specification of neural crest-derived sensory neurons. Development (Cambridge, England). 133(23):4619-4630
Roussigne, M., and Blader, P. (2006) Divergence in regulation of the PEA3 family of ETS transcription factors. Gene Expression Patterns. 6(8):777-782
Dufourcq, P., Roussigne, M., Blader, P., Rosa, F., Peyriéras, N., and Vriz, S. (2006) Mechano-sensory organ regeneration in adults: The zebrafish lateral line as a model. Molecular and Cellular Neuroscience. 33(2):180-187
Etard, C., Behra, M., Ertzer, R., Fischer, N., Jesuthasan, S., Blader, P., Geisler, R., and Strähle, U. (2005) Mutation in the delta-subunit of the nAChR suppresses the muscle defects caused by lack of Dystrophin. Developmental dynamics : an official publication of the American Association of Anatomists. 234(4):1016-1025
Knaut, H., Blader, P., Strähle, U., and Schier, A.F. (2005) Assembly of trigeminal sensory Ganglia by chemokine signaling. Neuron. 47(5):653-666
Blader, P., Lam, C.S., Rastegar, S., Scardigli, R., Nicod, J.C., Simplicio, N., Plessy, C., Fischer, N., Schuurmans, C., Guillemot, F., and Strähle, U. (2004) Conserved and acquired features of neurogenin1 regulation. Development (Cambridge, England). 131(22):5627-5637
Hans, S., Scheer, N., Riedl, I., Von Weizsacker, E., Blader, P., and Campos-Ortega, J.A. (2004) her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling. Development (Cambridge, England). 131(12):2957-2969
Dufourcq, P., Rastegar, S., Strähle, U., and Blader, P. (2004) Parapineal specific expression of gfi1 in the zebrafish epithalamus. Gene Expression Patterns. 4(1):53-57
Blader, P., Plessy, C., and Strähle, U. (2003) Multiple regulatory elements with spatially and temporally distinct activities control neurogenin1 expression in primary neurons of the zebrafish embryo. Mechanisms of Development. 120(2):211-218
Albert, S., Müller, F., Fischer, N., Biellmann, D., Neumann, C., Blader, P., and Strähle, U. (2003) Cyclops-independent floor plate differentiation in zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists. 226(1):59-66
Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U. (2002) A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2. Developmental Biology. 252(1):1-14
Müller, F., Blader, P., and Strähle, U. (2002) Search for enhancers: teleost models in comparative genomic and transgenic analysis of cis regulatory elements. BioEssays : news and reviews in molecular, cellular and developmental biology. 24(6):564-572
Dickmeis, T., Aanstad, P., Clark, M., Fischer, N., Herwig, R., Mourrain, P., Blader, P., Rosa, F., Lehrach, H., and Strähle, U. (2001) Identification of nodal signaling targets by array analysis of induced complex probes. Developmental dynamics : an official publication of the American Association of Anatomists. 222(4):571-580
Müller, F., Albert, S., Blader, P., Fischer, N., Hallonet, M., and Strähle, U. (2000) Direct action of the Nodal-related signal Cyclops in induction of sonic hedgehog in the ventral midline of the CNS. Development (Cambridge, England). 127(18):3889-3897
Blader, P. and Strähle, U. (2000) Zebrafish developmental genetics and central nervous system development. Hum. Mol. Genet.. 9(6):945-951
Müller, F., Blader, P., Rastegar, S., Fischer, N., Knochel, W., Strähle, U. (1999) Characterization of zebrafish smad1, smad2 and smad5: the amino-terminus of Smad1 and Smad5 is required for specific function in the embryo. Mechanisms of Development. 88(1):73-88
Hammerschmidt, M., Blader, P., and Strähle, U. (1999) Strategies to perturb zebrafish development. In The Zebrafish: Biology. H.W. Detrich, L.I. Zon, and M. Westerfield, eds. Methods Cell Biol.. 59:87-115
Blader, P. and Strähle, U. (1998) Ethanol impairs migration of the prechordal plate in the zebrafish embryo. Developmental Biology. 201:185-201
Blader, P. and Strähle, U. (1998) Casting an eye over cyclopia. Nature. 395:112-113
Blader, P., Rastegar, S., Fischer, N., and Strähle, U. (1997) Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid. Science (New York, N.Y.). 278(5345):1937-1940
Blader, P., Fischer, N., Gradwohl, G., Guillemot, F., and Strähle, U. (1997) The activity of Neurogenin1 is controlled by local cues in the zebrafish embryo. Development (Cambridge, England). 124:4557-4569
Chang, B.E., Blader, P., Fischer, N., Ingham, P.W., and Strähle, U. (1997) Axial (HNF3b) and retinoic acid receptors are regulators of the zebrafish sonic hedgehog promoter. The EMBO journal. 16(13):3955-3964
Strähle, U., Jesuthasan, S., Blader, P., Garcia-Villalba, P., Hatta, K., and Ingham, P.W. (1997) one-eyed pinhead is required for development of the ventral midline of the zebrafish (Danio rerio) neural tube. Genes and function. 1:131-148
Strähle, U., Fischer, N., and Blader, P. (1997) Expression and regulation of a netrin homologue in the zebrafish embryo. Mechanisms of Development. 62(2):147-160
Rauch, G.-J., Hammerschmidt, M., Blader, P., Schauerte, H.E., Strähle, U., Ingham, P.W., McMahon, A.P. and Haffter, P. (1997) WNT5 is required for tail formation in the zebrafish embryo. Cold Spring Harbor symposia on quantitative biology. 62:227-233
Strähle, U., Blader, P., and Ingham, P.W. (1996) Expression of axial and sonic hedgehog in wildtype and midline defective zebrafish embryos. The International journal of developmental biology. 40(5):929-940
Blader, P., Strähle, U., and Ingham, P.W. (1996) Three Wnt genes expressed in a wide variety of tissues during development of the zebrafish, Danio rerio: developmental and evolutionary perspectives. Development genes and evolution. 206(1):3-13
Strähle, U. and Blader, P. (1994) Early neurogenesis in the zebrafish embryo. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 8:692-698
Strähle, U., Blader, P., Adam, J., and Ingham, P.W. (1994) A simple and efficient procedure for non-isotopic in situ hybridization to sectioned material. Trends in genetics : TIG. 10:75-76
Strähle, U., Blader, P., Henrique, D., and Ingham, P.W. (1993) Axial, a zebrafish gene expressed along the developing body axis, shows altered expression in cyclops mutant embryos. Genes and Development. 7:1436-1446
Roussigné, M., Blader, P., and Wilson, S.W. The zebrafish epithalamus clears a path through the complexity of brain lateralization. Developmental Neurobiology.