PUBLICATION

Identification of nodal signaling targets by array analysis of induced complex probes

Authors
Dickmeis, T., Aanstad, P., Clark, M., Fischer, N., Herwig, R., Mourrain, P., Blader, P., Rosa, F., Lehrach, H., and Strähle, U.
ID
ZDB-PUB-011217-5
Date
2001
Source
Developmental dynamics : an official publication of the American Association of Anatomists   222(4): 571-580 (Journal)
Registered Authors
Aanstad, Pia, Blader, Patrick, Clark, Matthew D., Dickmeis, Thomas, Fischer, Nadine, Lehrach, Hans, Mourrain, Philippe, Rosa, Frederic, Strähle, Uwe
Keywords
organizer, mesoderm, endoderm, nodal, cyclops, squint, zebrafish, endoderm formation, CDNA libraries, zebrafish, expression, organizer, pathway, requires, gastrulation, mouse, trunk
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/physiology
  • Gastrula/physiology
  • Nodal Protein
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Signal Transduction/physiology*
  • Transforming Growth Factor beta/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
11748827 Full text @ Dev. Dyn.
Abstract
Nodal signaling controls germ layer formation, left-right asymmetry, and patterning of the brain in the vertebrate embryo. Cellular responses to Nodal signals are complex and include changes in gene expression, cell morphology, and migratory behavior. Only little is known about the genes regulated by Nodal signaling. We designed a subtractive screening strategy by using a constitutively active Nodal receptor to identify putative target genes of Nodal signals in the early gastrula of zebrafish embryos. By quantitative analysis of macro-array hybridizations, 132 genes corresponding to 1.4% of genes on the entire macro-array were identified, which were enriched in the Nodal-induced probe pool. These genes encode components of signal transduction pathways, transcription regulators, proteins involved in protein metabolism but also cytoskeletal components and metabolic enzymes, suggesting dramatic changes of cell physiology in gastrula cells in response to Nodal signals.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes