PUBLICATION

Pitx2c orchestrates embryonic axis extension via mesendodermal cell migration

Authors
Collins, M.M., Maischein, H.M., Dufourcq, P., Charpentier, M., Blader, P., Stainier, D.Y.
ID
ZDB-PUB-180629-6
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Blader, Patrick, Dufourcq, Pascale, Maischein, Hans-Martin, Stainier, Didier
Keywords
Pitx2c, cell migration, chemokine signaling, developmental biology, gastrulation, integrin-fibronectin interaction, zebrafish
Datasets
GEO:GSE114671
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/genetics
  • Cell Movement/genetics*
  • Cell Shape
  • Chemokines/genetics
  • Chemokines/metabolism
  • Embryo, Nonmammalian
  • Embryonic Development/genetics*
  • Endoderm/cytology
  • Endoderm/metabolism
  • Epithelial Cells/cytology
  • Epithelial Cells/metabolism*
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism
  • Gastrulation/genetics
  • Gene Expression Regulation, Developmental*
  • Integrins/genetics
  • Integrins/metabolism
  • Mutation
  • Notochord/cytology
  • Notochord/metabolism
  • Time-Lapse Imaging
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Transcriptome
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zygote/cytology
  • Zygote/growth & development
  • Zygote/metabolism
PubMed
29952749 Full text @ Elife
Abstract
Pitx2c, a homeodomain transcription factor, is classically known for its left-right patterning role. However, an early wave of pitx2 expression occurs at the onset of gastrulation in several species, indicating a possible earlier role that remains relatively unexplored. Here we show that in zebrafish, maternal-zygotic (MZ) pitx2c mutants exhibit a shortened body axis indicative of convergence and extension (CE) defects. Live imaging reveals that MZpitx2c mutants display less persistent mesendodermal migration during late stages of gastrulation. Transplant data indicate that Pitx2c functions cell non-autonomously to regulate this cell behavior by modulating cell shape and protrusive activity. Using transcriptomic analyses and candidate gene approaches, we identify transcriptional changes in components of the chemokine-ECM-integrin dependent mesendodermal migration network. Together, our results define pathways downstream of Pitx2c that are required during early embryogenesis and reveal novel functions for Pitx2c as a regulator of morphogenesis.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes