PUBLICATION

Search for enhancers: teleost models in comparative genomic and transgenic analysis of cis regulatory elements

Authors
Müller, F., Blader, P., and Strähle, U.
ID
ZDB-PUB-020716-17
Date
2002
Source
BioEssays : news and reviews in molecular, cellular and developmental biology   24(6): 564-572 (Review)
Registered Authors
Blader, Patrick, Müller, Ferenc, Strähle, Uwe
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Enhancer Elements, Genetic
  • Fishes
  • Humans
  • Internet
  • Molecular Sequence Data
  • Oryzias
  • Phylogeny
  • Transgenes*
  • Zebrafish
PubMed
12111739 Full text @ Bioessays
Abstract
Homology searches between DNA sequences of evolutionary distant species (phylogenetic footprinting) offer a fast detection method for regulatory sequences. Because of the small size of their genomes, tetraodontid species such as the Japanese pufferfish and green spotted pufferfish have become attractive models for comparative genomics. A disadvantage of the tetraodontid species is, however, that they cannot be bred and manipulated routinely under laboratory conditions, so these species are less attractive for developmental and genetic analysis. In contrast, an increasing arsenal of transgene techniques with the developmental model species zebrafish and medaka are being used for functional analysis of cis regulatory sequences. The main disadvantage is the much larger genome. While comparison between many loci proved the suitability of phylogenetic footprinting using fish and mammalian sequences, fast rate of change in enhancer structure and gene duplication within teleosts may obscure detection of homologies. Here we discuss the contribution and potentials provided by different teleost models for the detection and functional analysis of conserved cis-regulatory elements.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes