PUBLICATION
Ethanol impairs migration of the prechordal plate in the zebrafish embryo
- Authors
- Blader, P. and Strähle, U.
- ID
- ZDB-PUB-981006-4
- Date
- 1998
- Source
- Developmental Biology 201: 185-201 (Journal)
- Registered Authors
- Blader, Patrick, Strähle, Uwe
- Keywords
- cyclopia; goosecoid; islet-1; hgg1; prechordal plate mesoderm; fetal alcohol syndrome
- MeSH Terms
-
- Gastrula/drug effects
- Otx Transcription Factors
- Embryonic Development
- Blastocyst/drug effects
- Animals
- Embryo, Nonmammalian/drug effects
- Teratogens/pharmacology
- Hedgehog Proteins
- Gene Expression Regulation, Developmental/drug effects*
- Goosecoid Protein
- Colforsin/pharmacology
- Fetal Proteins/genetics
- Repressor Proteins*
- Zebrafish/embryology*
- Holoprosencephaly/physiopathology
- Transcription Factors/genetics
- Brain/drug effects*
- Forkhead Transcription Factors
- Microinjections
- RNA, Messenger/metabolism
- Proteins/genetics
- Cell Movement/drug effects*
- T-Box Domain Proteins*
- Ethanol/pharmacology*
- Nerve Tissue Proteins/metabolism
- Immunohistochemistry
- In Situ Hybridization
- Zebrafish Proteins*
- Homeodomain Proteins/genetics
- Trans-Activators/metabolism
- PubMed
- 9740658 Full text @ Dev. Biol.
Citation
Blader, P. and Strähle, U. (1998) Ethanol impairs migration of the prechordal plate in the zebrafish embryo. Developmental Biology. 201:185-201.
Abstract
Exposure of vertebrate embryos to ethanol causes cyclopia, but little is known about the underlying mechanisms of this effect. Here we show that cyclopia can be induced in the zebrafish by a short ethanol treatment during early gastrula stages and is accompanied by loss of gene expression characteristic of the ventral aspects of the fore- and midbrain. Interestingly, defects in the expression of ventral brain markers are linked to impaired migration of the prechordal plate mesoderm indicating that the correct position of the prechordal plate mesoderm under the anterior neural plate in the zebrafish embryo is required for specification of the anterior neural midline. Ethanol-induced cyclopia does not, however, impair the induction of anterior neuroectodermal structures in general. Finally, as genes like goosecoid and islet-1 are expressed in prechordal plate cells in a temporal pattern similar to control embryos despite the ectopic position of expressing cells, it appears that regulation of prechordal plate-specific gene expression is largely independent of the final position of the prechordal plate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping