PUBLICATION

Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm

Authors
Aguillon, R., Batut, J., Subramanian, A., Madelaine, R., Dufourcq, P., Schilling, T.F., Blader, P.
ID
ZDB-PUB-180103-5
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Batut, Julie, Blader, Patrick, Dufourcq, Pascale, Schilling, Tom, Subramanian, Arul
Keywords
developmental biology, lineage origin, neuronal subtype, olfactory placode, stem cells, zebrafish
MeSH Terms
  • Animals
  • Cell Lineage*
  • Ectoderm/embryology*
  • Microscopy, Confocal
  • Neurons/physiology*
  • Olfactory Mucosa/embryology*
  • Time-Lapse Imaging
  • Zebrafish/embryology*
PubMed
29292696 Full text @ Elife
Abstract
The zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory epithelium. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes