Fig. 2. The expression levels of angiogenesis-related genes were detected in zebrafish after MNU treatment. A. Transcriptome sequencing analysis of the expression levels of MNU-induced angiogenesis-related genes in zebrafish embryos. Genes significantly upregulated are shown in red, while genes that are significantly downregulated are shown in blue in heat map. Data are shown as the mean ± SEM. * P < 0.05, * * P < 0.01 and * ** P < 0.001. B. RT-qPCR data shows the expression levels of genes related to angiogenesis in normal embryos and MNU-treated embryos. Data are obtained from three independent experiments and shown as the mean ± SEM. * P < 0.05, * ** P < 0.01. C. Representative images of ISV and hindbrain vessels from control group, MNU-treated group, p53 MO-injected group and MNU-treated p53 MO group at 48 hpf. The hindbrain vessel images show the primary vessels, including primordial hind-brain channels (PHBC), basilar artery (BA), and central artery (CA). The blue arrow points to PHBC, the yellow arrow points to BA, and the red arrows point to CA. White arrows point to ectopic ISV branches. Scale bar, 50 µm. D. The percentage of ectopic ISV branches in control group, MNU-treated group, p53 MO-treated group and MNU-treated p53 MO group at 48 hpf. Data are obtained from three independent experiments. * ** P < 0.001.
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