ZFIN ID: ZDB-FIG-200612-29
Weinschutz Mendes et al., 2020 - Expression of dlx genes in the normal and regenerating brain of adult zebrafish. PLoS One   15:e0229549 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Adult
PHENOTYPE:
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Observed In:
Stage: Adult

Fig 5 Expression of <italic>dlx1a</italic>, <italic>dlx2a</italic>, <italic>dlx5a</italic> and <italic>dlx6a</italic> post-lesion in adult zebrafish.

Top left panel (A) shows the location of the mechanical lesion. Expression of the four dlx paralogs in controls (C-F and G-J) and lesioned brains at 3 dpl (C’-F’) and 7 dpl (G’-J’). Down-regulation of dlx5a is suggested at 3dpl (E’ indicated by gray arrow) and confirmed by RT-qPCR (B) (Student’s t-test, n = 5, p = 0.004). A slight up-regulation of dlx2a and dlx5a is seen at 7dpl compared to controls. For each gene, and for each time point, assays were carried out with at least 2 biological replicates in 3 different experimental repetitions (N = 6). RT-qPCR analyses at 7dpl (K) reveals no significant changes in expression levels of dlx1a, dlx2a and dlx6a (N = 7 for each gene each). A significant increase in dlx5a expression was observed at 7dpl (Student’s t-test, n = 7, p = 0.008). Scale bar: 400 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx1a WT standard conditions Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
WT surgical manipulation: telencephalon Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
dlx2a WT standard conditions Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
WT surgical manipulation: telencephalon Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
dlx5a WT standard conditions Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
WT surgical manipulation: telencephalon Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
dlx6a WT standard conditions Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
WT surgical manipulation: telencephalon Adult dorsal nucleus of ventral telencephalon ISH
Adult forebrain RTPCR
Adult ventral nucleus of ventral telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT surgical manipulation: telencephalon Adult dorsal nucleus of ventral telencephalon dlx5a expression decreased amount, abnormal
Adult forebrain dlx5a expression decreased amount, abnormal
Acknowledgments:
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