ZFIN Image: Weinschutz Mendes et al., 2020, Fig 5

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Figure Caption

Fig 5 Expression of <italic>dlx1a</italic>, <italic>dlx2a</italic>, <italic>dlx5a</italic> and <italic>dlx6a</italic> post-lesion in adult zebrafish.

Top left panel (A) shows the location of the mechanical lesion. Expression of the four dlx paralogs in controls (C-F and G-J) and lesioned brains at 3 dpl (C’-F’) and 7 dpl (G’-J’). Down-regulation of dlx5a is suggested at 3dpl (E’ indicated by gray arrow) and confirmed by RT-qPCR (B) (Student’s t-test, n = 5, p = 0.004). A slight up-regulation of dlx2a and dlx5a is seen at 7dpl compared to controls. For each gene, and for each time point, assays were carried out with at least 2 biological replicates in 3 different experimental repetitions (N = 6). RT-qPCR analyses at 7dpl (K) reveals no significant changes in expression levels of dlx1a, dlx2a and dlx6a (N = 7 for each gene each). A significant increase in dlx5a expression was observed at 7dpl (Student’s t-test, n = 7, p = 0.008). Scale bar: 400 μM.

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Acknowledgments

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