ZFIN ID: ZDB-FIG-190723-1350
Pociute et al., 2019 - Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish. BMC Developmental Biology   19:6 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3

Analysis of vascular marker expression by in situ hybridization at 24 hpf in clec14a−/− embryos injected with 10 ng of c1qr MO. a-d kdrl, e-h fli1a, i-l cdh5, m-p arterial marker cldn5b, q-t venous marker flt4expression. Note the strong inhibition of angiogenic sprouting in the double clec14a−/−; c1qr MO embryos (arrowheads). Only weak or partial inhibition of sprouting is observed in clec14a−/− or c1qrMO embryos. u Percentage of embryos with defects in ISV sprouting is greatly increased among c1qrMO; clec14a−/− embryos compared to c1qr MO or clec14a−/− embryos. n refers to the number of embryos analyzed for each marker. *p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from two independent experiments. Error bars show standard error

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT standard conditions Prim-5 axial vasculature ISH
WT + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
Prim-5 intersegmental vessel ISH
cldn5b WT standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-cd93 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
clec14aci15/ci15 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
fli1 WT standard conditions Prim-5 axial vasculature ISH
WT + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
Prim-5 intersegmental vessel ISH
flt4 WT standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
WT + MO1-cd93 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
clec14aci15/ci15 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
kdrl WT standard conditions Prim-5 axial vasculature ISH
WT + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 axial vasculature ISH
Prim-5 intersegmental vessel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
clec14aci15/ci15 + MO1-cd93 standard conditions Prim-5 intersegmental vessel cdh5 expression decreased amount, abnormal
Prim-5 intersegmental vessel cdh5 expression spatial pattern, abnormal
Prim-5 intersegmental vessel cldn5b expression decreased amount, abnormal
Prim-5 intersegmental vessel cldn5b expression spatial pattern, abnormal
Prim-5 intersegmental vessel fli1 expression decreased amount, abnormal
Prim-5 intersegmental vessel fli1 expression spatial pattern, abnormal
Prim-5 intersegmental vessel kdrl expression decreased amount, abnormal
Prim-5 intersegmental vessel kdrl expression spatial pattern, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.