ZFIN ID: ZDB-FIG-190723-1352
Pociute et al., 2019 - Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish. BMC Developmental Biology   19:6 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

clec14a mutation and vegfaa MO knockdown show synergistic effect in inhibiting intersegmental vessel (ISV) sprouting. a-d ISH analysis of kdrl expression at 24 hpf. e-h Analysis of kdrl: GFP expression at 48 hpf. Note that partial inhibition of sprouting is observed using low dose of 2.5 ng vegfaa MO in wt embryos (c, g). The same dose in clec14a mutants results in a complete loss of sprouting at 24 hpf and significant inhibition of sprouting at 48 hpf (d, h). i Percentage of embryos with normal or inhibited ISVs based on kdrl expression at 24 hpf. *** p < 0.001, Fisher’s exact test; embryos with partially reduced ISVs were compared between wt and clec14a−/− or vegfaa MO groups; embryos with no ISVs were compared between the double knockdown vegfaa MO; clec14a−/− and the other groups. Data were combined from two independent experiments

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kdrl WT standard conditions Prim-5 axial vasculature ISH
WT + MO1-vegfaa standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 standard conditions Prim-5 axial vasculature ISH
clec14aci15/ci15 + MO1-vegfaa standard conditions Prim-5 axial vasculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vegfaa standard conditions Prim-5 axial vasculature kdrl expression spatial pattern, abnormal
clec14aci15/ci15 standard conditions Prim-5 axial vasculature kdrl expression spatial pattern, abnormal
clec14aci15/ci15 + MO1-vegfaa standard conditions Prim-5 axial vasculature kdrl expression spatial pattern, abnormal
clec14aci15/ci15; s843Tg standard conditions Long-pec intersegmental vessel angiogenic sprout decreased length, abnormal
Long-pec intersegmental vessel decreased amount, abnormal
Long-pec sprouting angiogenesis delayed, abnormal
clec14aci15/ci15; s843Tg + MO1-vegfaa standard conditions Long-pec dorsal longitudinal anastomotic vessel immature, abnormal
Long-pec intersegmental vessel angiogenic sprout decreased length, exacerbated
Long-pec intersegmental vessel decreased amount, exacerbated
Long-pec intersegmental vessel decreased length, abnormal
Long-pec parachordal vessel incomplete structure, exacerbated
Long-pec sprouting angiogenesis delayed, exacerbated
s843Tg + MO1-vegfaa standard conditions Long-pec intersegmental vessel angiogenic sprout decreased length, abnormal
Long-pec intersegmental vessel decreased amount, abnormal
Long-pec parachordal vessel incomplete structure, abnormal
Long-pec sprouting angiogenesis delayed, abnormal
Acknowledgments:
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