ZFIN ID: ZDB-FIG-190723-1348
Pociute et al., 2019 - Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish. BMC Developmental Biology   19:6 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: 26+ somites
PHENOTYPE:
Fish:
Observed In:
Stage: 26+ somites

Fig. 1

Generation of clec14a mutants by TALEN-mediated genome editing. a Wild-type and clec14aci15 mutant sequence showing the binding regions for TALE nucleases and the 10 bp deletion in the clec14a mutants which includes the deletion of HhaI restriction site used for genotyping. b A diagram of Clec14a protein sequence which includes C-type lectin and transmembrane (TM) domains. clec14aci15 mutation is predicted to cause a frameshift early in the protein coding sequence starting at the amino acid 44 and would lead to a premature stop codon. c, d DNA sequencing chromatogram shows a 10 bp deletion (boxed-in wild-type sequence) in the cDNA of clec14a mutants. d, e In situ hybridization analysis for clec14a expression in wt embryos (d) and clec14a mutants (e) at 24 hpf. Note a significant reduction of clec14a expression in clec14a mutant embryos. The experiment has been replicated twice; the combined number of embryos analyzed and showing the phenotype is shown in the lower right corner

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
clec14a WT standard conditions 26+ somites intermediate cell mass of mesoderm ISH
clec14aci15/ci15 standard conditions 26+ somites axial vasculature ISH
26+ somites intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
clec14aci15/ci15 standard conditions 26+ somites intermediate cell mass of mesoderm clec14a expression decreased amount, abnormal
Acknowledgments:
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