ZFIN ID: ZDB-FIG-180827-50
Zhao et al., 2018 - Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish. Nature communications   9:2639 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 4

Intestinal microorganisms are not required for PIK3C3 deficiency-induced inflammation. a Scatter plots showing the qRT-PCR results of the indicated genes in 8 dpf embryos under either standard or germ-free culture conditions. Each dot represents the mean of three technical replicates from one embryo. Inflammatory genes are significantly stimulated in mutants under germ-free culture condition. NS, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 in one-way ANOVA with Tukey’s multiple comparison. b Neutrophils accumulate in the digestive tract of mutant in the absence of gut microbiota. Images were acquired as described in Fig. 3c. c Scatter plot showing the numbers of bacteria in intestinal homogenates from individual embryo at 6, 7, and 8 dpf under standard culture condition. WT and mutants have similar gut bacteria at 6 and 7 dpf. However, the number of gut bacteria becomes either very low (<20 CFU/gut, 5 out of 14 embryos) or undetectable (9 out of 14) in the mutants at 8 dpf, which is significantly reduced when compared to that in wild type siblings (NS, non-significant; ***p < 0.001 in one-way ANOVA with Tukey’s multiple comparison)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cxcl8a TU standard conditions Days 7-13 Not Detected whole organism RTPCR
TU germ free Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism RTPCR
EGFP pik3c3zf2160/zf2160; rj30Tg germ free Days 7-13 intestine neutrophil IFL
pik3c3zf2161/zf2161; rj30Tg germ free Days 7-13 intestine neutrophil IFL
rj30Tg germ free Days 7-13 intestine neutrophil IFL
fabp2 TU standard conditions Days 7-13 whole organism RTPCR
TU germ free Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 Not Detected whole organism RTPCR
il1b TU standard conditions Days 7-13 Not Detected whole organism RTPCR
TU germ free Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism RTPCR
mmp9 TU standard conditions Days 7-13 Not Detected whole organism RTPCR
TU germ free Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism RTPCR
mpx TU standard conditions Days 7-13 whole organism RTPCR
TU germ free Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism RTPCR
tnfa TU standard conditions Days 7-13 Not Detected whole organism RTPCR
TU germ free Days 7-13 Not Detected whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pik3c3zf2160/zf2160 standard conditions Days 7-13 whole organism fabp2 expression absent, abnormal
Days 7-13 whole organism mpx expression increased amount, abnormal
pik3c3zf2160/zf2160 germ free Days 7-13 whole organism cxcl8a expression increased amount, abnormal
Days 7-13 whole organism fabp2 expression absent, abnormal
Days 7-13 whole organism il1b expression increased amount, abnormal
Days 7-13 whole organism mmp9 expression increased amount, abnormal
Days 7-13 whole organism mpx expression amount, ameliorated
Days 7-13 whole organism tnfa expression increased amount, abnormal
pik3c3zf2160/zf2160; rj30Tg germ free Days 7-13 intestinal epithelium has extra parts of type neutrophil, abnormal
Days 7-13 intestine neutrophil EGFP expression increased amount, abnormal
pik3c3zf2161/zf2161 standard conditions Days 7-13 whole organism fabp2 expression absent, abnormal
Days 7-13 whole organism mpx expression increased amount, abnormal
pik3c3zf2161/zf2161 germ free Days 7-13 whole organism cxcl8a expression increased amount, abnormal
Days 7-13 whole organism fabp2 expression absent, abnormal
Days 7-13 whole organism il1b expression increased amount, abnormal
Days 7-13 whole organism mmp9 expression increased amount, abnormal
Days 7-13 whole organism mpx expression amount, ameliorated
Days 7-13 whole organism tnfa expression increased amount, abnormal
pik3c3zf2161/zf2161; rj30Tg germ free Days 7-13 intestinal epithelium has extra parts of type neutrophil, abnormal
Days 7-13 intestine neutrophil EGFP expression increased amount, abnormal
Acknowledgments:
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