ZFIN ID: ZDB-FIG-180827-48
Zhao et al., 2018 - Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish. Nature communications   9:2639 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 6 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 2

Defective gene expressions in pik3c3 mutants. a RNA-Seq analysis of pik3c3 mutants and control siblings at 6–8 dpf. Representative heatmaps show the expression of genes in one of these categories: extracellular matrix-related genes, liver and gut metabolic genes or markers of endoderm-derived cell lineages. b qRT-PCR analysis of the indicated genes from whole embryonic extracts. apoa1a is an apolipoprotein family member expressed in liver, gut, and other tissues. fabp2 is intestinal fatty acid-binding protein. villin 1 (vil1) and slc15a1b (pept1) are markers of intestinal epithelium. fabp10a is liver fatty acid-binding protein. trypsin (try) and insulin (ins) are markers of exocrine and endocrine pancreas, respectively. gapdh is the internal control and data represent mean ± SD from three independent biological repeats (NS, non-significant; *p < 0.05; **p < 0.01; ****p < 0.0001 in one-way ANOVA with Tukey’s multiple comparison). c In situ hybridization of 8 dpf embryos with the indicated probes. Red arrows point to intestines and black arrows indicate pancreases. Numbers represent (embryos with the indicated phenotype)/(total embryos analyzed)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoa1a TU standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
fabp2 TU standard conditions Day 6 whole organism RTPCR
Days 7-13 intestine ISH
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 intestine ISH
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 intestine RTPCR
Days 7-13 intestine ISH
fabp10a TU standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
ins TU standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
prss1 TU standard conditions Day 6 whole organism RTPCR
Days 7-13 exocrine pancreas ISH
Days 7-13 whole organism RTPCR
Days 7-13 whole organism ISH
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 exocrine pancreas ISH
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 exocrine pancreas ISH
Days 7-13 whole organism RTPCR
slc15a1b TU standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
vil1 TU standard conditions Day 6 whole organism RTPCR
Days 7-13 intestinal epithelium ISH
Days 7-13 whole organism RTPCR
pik3c3zf2160/zf2160 standard conditions Day 6 whole organism RTPCR
Days 7-13 intestinal epithelium ISH
Days 7-13 whole organism RTPCR
pik3c3zf2161/zf2161 standard conditions Day 6 whole organism RTPCR
Days 7-13 intestinal epithelium ISH
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pik3c3zf2160/zf2160 standard conditions Days 7-13 intestinal epithelium vil1 expression decreased amount, abnormal
Days 7-13 whole organism apoa1a expression decreased amount, abnormal
Days 7-13 whole organism fabp2 expression decreased amount, abnormal
Days 7-13 whole organism prss1 expression increased amount, abnormal
Days 7-13 whole organism slc15a1b expression decreased amount, abnormal
Days 7-13 whole organism vil1 expression decreased amount, abnormal
pik3c3zf2161/zf2161 standard conditions Days 7-13 intestinal epithelium vil1 expression decreased amount, abnormal
Days 7-13 intestine fabp2 expression decreased amount, abnormal
Days 7-13 intestine fabp2 expression decreased amount, abnormal
Days 7-13 whole organism apoa1a expression decreased amount, abnormal
Days 7-13 whole organism prss1 expression increased amount, abnormal
Days 7-13 whole organism slc15a1b expression decreased amount, abnormal
Days 7-13 whole organism vil1 expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.