ZFIN ID: ZDB-FIG-180827-47
Zhao et al., 2018 - Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish. Nature communications   9:2639 Full text @ Nat. Commun.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 6 to Days 7-13

Fig. 1

Intestinal defects in pik3c3 mutants. a General morphology of WT and pik3c3 mutants at 8 dpf. b In vivo imaging of zebrafish digestive organ with fluorescent reporters. FITC-labeled dextran is used to evaluate the nutrient uptake activity and the quenched fluorescent reporter PED6 or Enzchek is activated only after cleavage by intestinal phospholipase or protease. The digestive functions appear normal in mutants at 7 dpf while they become defective at 8 dpf. Scale bars, 500 µm. Numbers in (a, b) represent (embryos with the indicated phenotype)/(total embryos analyzed). c Hematoxylin–eosin staining of cross sections corresponding to the intestinal bulb or mid-intestine of embryos at 7–8 dpf. Typical intestinal folding is lost and cell shedding can be detected in pik3c3 mutants at 8 dpf. Scale bars, 50 µm. d TEM analyses of IECs. Adherens junctions (arrow) are defective in mutants. Microvilli and cell junctions are severely disrupted in mutants at 8 dpf. Scale bars, 1 µm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pik3c3zf2160/zf2160 standard conditions Day 6 digestion process efficacy, normal
Day 6 intestinal bulb has normal numbers of parts of type intestinal villus, normal
Day 6 mid intestine has normal numbers of parts of type intestinal villus, normal
Days 7-13 digestion decreased efficacy, abnormal
Days 7-13 intestinal bulb lacks parts or has fewer parts of type intestinal villus, abnormal
Days 7-13 intestinal epithelial structure maintenance disrupted, abnormal
Days 7-13 intestinal epithelium adherens junction incomplete structure, abnormal
Days 7-13 intestinal epithelium microvillus absent, abnormal
Days 7-13 mid intestine lacks parts or has fewer parts of type intestinal villus, abnormal
Days 7-13 whole organism bent, abnormal
pik3c3zf2161/zf2161 standard conditions Day 6 digestion process efficacy, normal
Day 6 intestinal bulb has normal numbers of parts of type intestinal villus, normal
Day 6 mid intestine has normal numbers of parts of type intestinal villus, normal
Days 7-13 digestion decreased efficacy, abnormal
Days 7-13 intestinal bulb lacks parts or has fewer parts of type intestinal villus, abnormal
Days 7-13 intestinal epithelial structure maintenance disrupted, abnormal
Days 7-13 intestinal epithelium adherens junction incomplete structure, abnormal
Days 7-13 intestinal epithelium microvillus absent, abnormal
Days 7-13 mid intestine lacks parts or has fewer parts of type intestinal villus, abnormal
Days 7-13 whole organism bent, abnormal
Acknowledgments:
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