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ZFIN ID: ZDB-FIG-180312-4
Li et al., 2017 - Rapid functional analysis of computationally complex rare human IRF6 gene variants using a novel zebrafish model. PLoS Genetics   13:e1007009 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Sphere
PHENOTYPE:
Fish:
Observed In:
Stage: Sphere

Fig. 3

Maternal-null irf6-/- embryo gene expression is rescued by injection of either zebrafish or human wild type IRF6 mRNA.

(A-H) Whole-mount in situ hybridization analysis of wild type embryos (A-D) compared to maternal/zygotic-null irf6 -/- embryos (E-H) at the sphere stage revealed strong down-regulation of critical irf6 downstream genes such as krt4 and grhl3. Scale bar = 150 μm. (I) Relative gene expression of wild type embryos, maternal/zygotic-null irf6 -/- embryos, and maternal/zygotic-null irf6 -/- embryos rescued with either wild type zebrafish or human IRF6 mRNA (100 pg) microinjections, for a panel of genes with crucial roles in the irf6 gene regulatory network. Error bar = 2xSEM, n = 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cebpb irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
cldne irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
gata3 irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
grhl1 irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
grhl3 TU standard conditions Sphere whole organism ISH
irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
Sphere Not Detected whole organism ISH
hey1 irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
klf2a TU standard conditions Sphere whole organism ISH
irf6zf2064(TU) standard conditions Sphere Not Detected whole organism ISH
Sphere whole organism RTPCR
krt4 TU standard conditions Sphere whole organism ISH
irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
Sphere Not Detected whole organism ISH
krt18a.1 TU standard conditions Sphere whole organism ISH
irf6zf2064(TU) standard conditions Sphere Not Detected whole organism ISH
tfap2a irf6zf2064(TU) standard conditions Sphere whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
irf6zf2064(TU) standard conditions Sphere whole organism cebpb expression decreased amount, abnormal
Sphere whole organism gata3 expression decreased amount, abnormal
Sphere whole organism cldne expression decreased amount, abnormal
Sphere whole organism hey1 expression decreased amount, abnormal
Sphere whole organism klf2a expression decreased amount, abnormal
Sphere whole organism grhl1 expression decreased amount, abnormal
Sphere whole organism grhl3 expression decreased amount, abnormal
Sphere whole organism tfap2a expression decreased amount, abnormal
Sphere whole organism krt4 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.