Fig. S6

Normal expressions of various neuronal markers in sam2^cnu1 KO fish. (A) Alignment of human, mouse, and zebrafish Sam2 peptides. Putative amino acid sequences of mutant forms are also aligned. Dopaminergic neuron (th, B-B’; nurr1, C-C’; dat, L-L’), serotonergic neuron (tphR, D-D’; 5-HT, G-G’; pet-1, M-M’), oxytocinergic neuron (oxt, E-E’), and IPN (sst1, F-F’) markers. Additional neural markers: hcrt (H-H’), mch (I-I’), agrp (J-J’), and npy (K-K’). (N-O’) Normal hypothalamus (H) - locus coeruleus (LC) axonal connections in sam2^cnu1 KO fish as judged by Tg[hcrt:EGFP] transgene expression. Dorsal views, anterior to the top. Scale bar, 100 μm. (P-R’) Serotonergic neuron (tphR, slc6a4a) and adrenocorticotropic hormone precursor pomc marker in adult brain. Arrowheads indicate the ventral posterior tuberculum (P-Q’) and the nucleus lateralis tuberis (R, R’). Bracket defines the raphe (P-Q’). (S-S’) c-fos expression in the novel tank assay. Scale bar, 500 μm.