ZFIN ID: ZDB-FIG-180207-7
Choi et al., 2018 - Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proceedings of the National Academy of Sciences of the United States of America   115(5):E1041-E1050 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 2

Generation of sam2 KO fish using ZFNs. (A) DNA sequencing analysis of two sam2 KO alleles (sam2cnu1 and sam2cnu2) with 5-bp and 17-bp deletion, respectively. Yellow mark, Left and Right ZFN-binding regions; red, spacer. (B and B′) Allele-specific genotyping of sam2cnu1 and sam2cnu2 KO fish by genomic PCR and digestion for BtgI site (CCGTGG) newly created in sam2cnu1. (C and C′) Normal morphology of sam2cnu1 KO embryo at 3 d. (D–E′) Projections of Hb efferent axons targeting the IPN in the Et(-1.0otpa:mmGFP)hd1 transgenic sam2cnu1 KO fish were not visibly affected. Lateral views (D and E) and dorsal views (D′ and E′). dIPN, dorsal IPN; FR, fasciculus retroflexus; lFR, Left FR; lHb, Left Hb; MR, median raphe; rFR, Right FR; rHb, Right HbvIPN, ventral IPN. (Scale bar, 100 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP hd1Tg standard conditions Protruding-mouth habenula axon IFL
sam2ck001a/ck001a; hd1Tg standard conditions Protruding-mouth habenula axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sam2ck001a/ck001a standard conditions Adult whole organism morphology, normal
sam2ck001b/ck001b standard conditions Adult whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA