ZFIN ID: ZDB-FIG-180207-8
Choi et al., 2018 - Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proceedings of the National Academy of Sciences of the United States of America   115(5):E1041-E1050 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 6

Increase of mRNA expression of stress-related crhb in sam2cnu1 KO fish and spontaneous inhibitory postsynaptic currents onto CRH neurons by SAM2. (A–D) Increase of crhb mRNA expression in sam2cnu1 KO fish (sam2+/+, n = 6; sam2−/−, n = 6). Ventral views of the whole brain of control sam2+/+ (A and C) and sam2−/− KO fish (B and D). (A′ and B′) Higher magnifications of the boxed regions in A and B are the PPa in zebrafish, homologous to the mouse PVN. (Scale bars, 200 µm.) (E) Representative photomicrograph of the Cre-dependent TdTomato in the PVN CRH neurons. (Magnification: E, 10×.) (F) Representative voltage-clamp traces of sIPSCs in response to SAM2 application in PVN CRH neurons. (G) SAM2 application significantly increased sIPSC frequency. (H) SAM2 did not affect sIPSC amplitude. (I) Representative voltage-clamp trace of eIPSC onto CRH neurons in response to SAM2 application. (J) SAM2 did not affect the amplitude of eIPSCs. (K) SAM2 did not change the paired-pulse ratio. *P < 0.05; **P < 0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crhb WT stress Protruding-mouth hypothalamus preoptic area ISH
sam2ck001a/ck001a stress Protruding-mouth hypothalamus preoptic area ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sam2ck001a/ck001a stress Protruding-mouth hypothalamus preoptic area crhb expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA