ZFIN Gene: slc6a3

ZFIN ID: ZDB-GENE-010316-1

ZFIN ID: ZDB-GENE-010316-1

Gene Name:

solute carrier family 6 member 3

Gene Symbol:

slc6a3 Nomenclature History

Previous Names:

DAT (1), dat (1), dopamine transporter (1)

Gene Type:

protein_coding_gene

Location:

Chr: 16 Mapping Details/Browsers

Description:

Predicted to have neurotransmitter:sodium symporter activity. Predicted to localize to integral component of membrane. Is expressed in several structures, including dopaminergic neurons; forebrain; male organism; medulla oblongata; and retina. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder; nicotine dependence; and obesity. Orthologous to human SLC6A3 (solute carrier family 6 member 3).

Genome Resources:

Alliance (1), Gene:80787 (1), Ensembl(GRCz11):ENSDARG00000004219 (2)

GENE EXPRESSION
All Expression Data:	58 figures from 45 publications
Wild-type Stages, Structures:	Zygote:1-cell (0.0h-0.75h) to Adult (90d-730d, breeding adult)
	caudal parvocellular preoptic nucleus caudal periventricular hypothalamus caudal tuberal nucleus caudal tuberculum dopaminergic neuron caudal tuberculum diencephalon dopaminergic neuron diencephalon ventral region diencephalon dopaminergic neuron epiphysis female organism griseum centrale hypothalamus magnocellular preoptic nucleus male organism midline column nucleus of the lateral recess nucleus of the posterior recess olfactory bulb paraventricular organ periventricular nucleus of caudal tuberculum dopaminergic neuron preoptic area dopaminergic neuron preoptic area pretectum dopaminergic neuron pretectum retina telencephalon unspecified ventral telencephalon dopaminergic neuron ventral thalamus dopaminergic neuron ventrolateral thalamic nucleus ventromedial thalamic nucleus whole organism
High Throughput Expression:	GEO (1) , Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
f8	Small Deletion	Unknown	Unknown	ZINC_FINGER_NUCLEASE
zf1089	Insertion	Exon 12	Frameshift	ZINC_FINGER_NUCLEASE

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
CRISPR1-slc6a3		1
CRISPR2-slc6a3		2
CRISPR3-slc6a3		1
MO1-slc6a3	N/A	1
TALEN1-slc6a3		2

PHENOTYPE

Data:	6 figures from 3 publications
Observed in:	adult behavior behavior whole organism

DISEASE ASSOCIATED WITH slc6a3 HUMAN ORTHOLOG

Disease Ontology Term	Multi-Species Data	OMIM Term	OMIM Phenotype ID
		Parkinsonism-dystonia, infantile, 1	613135
		{Nicotine dependence, protection against}	188890

DISEASE ASSOCIATED WITH slc6a3 VIA EXPERIMENTAL MODELS No data available

GENE ONTOLOGY

Ontology	GO Term
Biological Process	dopamine uptake involved in synaptic transmission (more)
Cellular Component	integral component of membrane (more)
Molecular Function	neurotransmitter:sodium symporter activity (more)

GO Terms (all 12)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS No data available

INTERACTIONS AND PATHWAYS

ANTIBODIES No data available

PLASMIDS

Addgene:Dat - EGFP - Tol2

CONSTRUCTS WITH SEQUENCES FROM slc6a3

Construct	Regulatory Regions	Coding Sequences	Species	Tg Lines	Publications
Tg(-7slc6a3:EGFP)	slc6a3	EGFP	Danio rerio	1	2
Tg(-7slc6a3:mRFP)	slc6a3	mRFP	Danio rerio	2	2
Tg(-11slc6a3:EGFP)	slc6a3	EGFP	Danio rerio	2	3
Tg(slc6a3:CFP-NTR)	slc6a3	CFP, NTR	Danio rerio	1	1
Tg(slc6a3:Cr.ChR2-ECFP)	slc6a3	ECFP	Chlamydomonas reinha, Danio rerio	1	1
Tg(slc6a3:EGFP)	slc6a3	EGFP	Danio rerio	1	16
Tg(slc6a3:GAL4-VP16)	slc6a3	GAL4, VP16	Danio rerio	1	2
Tg(slc6a3:MITO-mCherry-NPT)	slc6a3	mCherry, MITO, NPT	Danio rerio	1	2

MARKER RELATIONSHIPS No data available

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_131755 (1)	1890 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Polypeptide	UniProtKB:F1QGM4 (1)	629 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 7)

ORTHOLOGY for slc6a3 ( Chr: 16 )

CITATIONS (106)

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