ZFIN ID: ZDB-FIG-170925-24
Xie et al., 2017 - Lef1-dependent hypothalamic neurogenesis inhibits anxiety. PLoS Biology   15:e2002257 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 2

Lef1 activates expression of zebrafish hypothalamic genes associated with anxiety.

(A) Volcano plot of zebrafish RNA sequencing (RNA-seq) shows differentially expressed genes in the 3 days post-fertilization (dpf) hypothalamus of lef1 mutants compared to control. Only genes with adjusted P value (AdjP) <0.1 (green line) are shown. Genes with an absolute value of log2 ratio >1 (blue lines) are shown in red; others are shown in black. Node size represents the averaged fragments per kilobase of transcript per million mapped reads of a gene in the control. (B) Ingenuity Pathway Analysis (IPA) for zebrafish hypothalamic Lef1-dependent genes revealed 20 genes associated with anxiety and depressive disorder, listed in the table. (C and D) Representative images of whole mount in situ hybridization on 3 dpf control and lef1 mutant embryos for known Wnt targets (C) and genes associated with anxiety and depressive disorder (D). Red and yellow arrows indicate expression in caudal and rostral hypothalamus, respectively. Lateral (axin2, dkk1b, lef1, notum1a, crhbp, and grin2cb) or ventral (other genes) views were selected for optimal expression visualization. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Protruding-mouth central nervous system ISH
lef1zd11/zd11 standard conditions Protruding-mouth central nervous system ISH
crhbp WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
dkk1b WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
grin2cb WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
htr1ab WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
lef1 WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
notum1a WT standard conditions Protruding-mouth central nervous system ISH
lef1zd11/zd11 standard conditions Protruding-mouth central nervous system ISH
penka WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
si:dkey-219c10.4 WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
slc6a4b WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
sp5a WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
sp5l WT standard conditions Protruding-mouth brain ISH
lef1zd11/zd11 standard conditions Protruding-mouth brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lef1zd11/zd11 standard conditions Protruding-mouth brain si:dkey-219c10.4 expression decreased amount, abnormal
Protruding-mouth brain penka expression decreased amount, abnormal
Protruding-mouth brain htr1ab expression decreased amount, abnormal
Protruding-mouth brain sp5l expression decreased amount, abnormal
Protruding-mouth brain slc6a4b expression decreased amount, abnormal
Protruding-mouth brain sp5a expression decreased amount, abnormal
Protruding-mouth brain lef1 expression decreased amount, abnormal
Protruding-mouth brain crhbp expression decreased amount, abnormal
Protruding-mouth brain dkk1b expression decreased amount, abnormal
Protruding-mouth brain grin2cb expression decreased amount, abnormal
Protruding-mouth central nervous system notum1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.