header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-170227-7
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 7

Immunohistochemical analysis of cone photoreceptors in ahi1lri46 mutants. (A, B, E, F, I, J) Red-green double cones are labeled with zpr1 (red). Cone inner segments are preserved at 5 dpf and 3 months of age, but missing (arrowheads) at 5 months. (C, D, G, H, K, L) PNA staining (red) revealed that mutants had shorter cone outer segments ([D], arrowheads) at 5 dpf and missing at 5 months ([L], arrowheads). (M) Quantification of cone outer segment lengths between wild-type and ahi1lri46 mutants at 5 dpf. ***P < 0.001. N, quantification of cone density at 5 dpf.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Day 5 retinal cone cell cell body
IHC Day 5 retinal cone cell photoreceptor inner segment
IHC Days 45-89 retinal cone cell cell body
IHC Days 45-89 retinal cone cell photoreceptor inner segment
IHC Adult retinal cone cell cell body
IHC Adult retinal cone cell photoreceptor inner segment
IHC ahi1lri46/lri46 standard conditions Day 5 retinal cone cell cell body
IHC Day 5 retinal cone cell photoreceptor inner segment
IHC Days 45-89 retinal cone cell cell body
IHC Days 45-89 retinal cone cell photoreceptor inner segment
IHC Adult retinal cone cell cell body
IHC Adult retinal cone cell photoreceptor inner segment
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Day 5 retinal cone cell development decreased process quality, abnormal
Day 5 retinal cone cell photoreceptor cell outer segment organization decreased process quality, abnormal
Day 5 retinal cone cell photoreceptor outer segment decreased length, abnormal
Day 5 retinal neural layer has normal numbers of parts of type retinal cone cell, normal
Adult retinal cone cell lacks all parts of type retinal cone cell photoreceptor outer segment, abnormal
Adult retinal cone cell development decreased process quality, abnormal
Adult retinal cone cell photoreceptor cell outer segment organization decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.