ZFIN ID: ZDB-FIG-170227-7
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 7

Immunohistochemical analysis of cone photoreceptors in ahi1lri46 mutants. (A, B, E, F, I, J) Red-green double cones are labeled with zpr1 (red). Cone inner segments are preserved at 5 dpf and 3 months of age, but missing (arrowheads) at 5 months. (C, D, G, H, K, L) PNA staining (red) revealed that mutants had shorter cone outer segments ([D], arrowheads) at 5 dpf and missing at 5 months ([L], arrowheads). (M) Quantification of cone outer segment lengths between wild-type and ahi1lri46 mutants at 5 dpf. ***P < 0.001. N, quantification of cone density at 5 dpf.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Day 5 retinal cone cell cell body
IHC Day 5 retinal cone cell photoreceptor inner segment
IHC Days 45-89 retinal cone cell cell body
IHC Days 45-89 retinal cone cell photoreceptor inner segment
IHC Adult retinal cone cell cell body
IHC Adult retinal cone cell photoreceptor inner segment
IHC ahi1lri46/lri46 standard conditions Day 5 retinal cone cell cell body
IHC Day 5 retinal cone cell photoreceptor inner segment
IHC Days 45-89 retinal cone cell cell body
IHC Days 45-89 retinal cone cell photoreceptor inner segment
IHC Adult retinal cone cell cell body
IHC Adult retinal cone cell photoreceptor inner segment
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Day 5 retinal cone cell development decreased process quality, abnormal
ahi1lri46/lri46 standard conditions Day 5 retinal cone cell photoreceptor cell outer segment organization decreased process quality, abnormal
ahi1lri46/lri46 standard conditions Day 5 retinal cone cell photoreceptor outer segment decreased length, abnormal
ahi1lri46/lri46 standard conditions Day 5 retinal neural layer has normal numbers of parts of type retinal cone cell, normal
ahi1lri46/lri46 standard conditions Adult retinal cone cell lacks all parts of type retinal cone cell photoreceptor outer segment, abnormal
ahi1lri46/lri46 standard conditions Adult retinal cone cell development decreased process quality, abnormal
ahi1lri46/lri46 standard conditions Adult retinal cone cell photoreceptor cell outer segment organization decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.