ZFIN ID: ZDB-FIG-170227-5
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 5

Ultrastructural analysis of wild-type and ahi1lri46 photoreceptors. (A, B) Transmission electron microscopy provided ultrastructural views of wild-type and ahi1lri46 photoreceptors. Mutant outer segments are short, fragmented, and disorganized. (C) Higher magnification of wild-type photoreceptor showing tightly stacked disc membranes and inner segment. (D) Vesiculation at the proximal end of an outer segment in ahi1lri46 mutant (arrowhead). (E) Improper orientation of disc membranes. In this field, an axoneme (white arrow) and disc membranes from outer segments of three different cells, labeled 1 to 3, are oriented perpendicular to each other. (F) Several layers of discs are abnormally wrapped around the outer segment (white arrow). (G, H) Cross-sections through the ciliary pocket (cp) show the proper 9 + 0 microtubule arrangement of axoneme. Mitochondria (m) are visible in (G). Y-shaped crosslinkers also are present (arrows in [G, H]). Staining artifacts are visible as diffuse, black deposits in some panels. Scale bars: 1 μm (A–F) and 200 nm (G, H).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Day 5 retinal cone cell axoneme morphology, normal
Day 5 retinal cone cell photoreceptor cell outer segment organization decreased process quality, abnormal
Day 5 retinal cone cell photoreceptor disc membrane irregular spatial pattern, abnormal
Day 5 retinal cone cell photoreceptor outer segment decreased length, abnormal
Day 5 retinal cone cell photoreceptor outer segment disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.