ZFIN ID: ZDB-FIG-170227-2
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 2

Loss of ahi1 affects other ciliated tissues. (A, B) Anti-acetylated tubulin whole-mount immunostaining (green) of kidney duct in wild-type and ahi1lri46 mutants at 36 hpf. Anterior is to the left in both images. Cilia line the pronephric duct (small arrowheads) in wild-type animals. The contralateral duct also can be observed (small arrows). Cilia are absent in the middle and distal pronephros in ahi1lri46 mutants (arrowhead in [B] indicates the proximal boundary where cilia are missing). (C, D) In the proximal region, mutant cilia are not qualitatively different from wild-type controls. (E, F) Cilia in the distal region of the pronephros are absent in mutant animals when compared to controls. Ift88 immunostaining (red) was used to mark the ciliary transition zone. Scale bars: 50 μm (A, B) and 20 μm (C–F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ift88 Ab1-ift88 WT standard conditions Prim-25 pronephric duct ciliary transition zone IHC
Ab1-ift88 ahi1lri46/lri46 standard conditions Prim-25 pronephric duct ciliary transition zone IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tuba IHC WT standard conditions Prim-25 pronephric duct motile cilium
IHC ahi1lri46/lri46 standard conditions Prim-25 Not Detected posterior pronephric duct motile cilium
IHC Prim-25 pronephric duct motile cilium
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Prim-25 posterior pronephric duct lacks all parts of type posterior pronephric duct motile cilium, abnormal
Prim-25 pronephric duct cilium assembly decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.