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ZFIN ID: ZDB-FIG-170227-8
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 9

ahi1 is not required for ciliogenesis or transition zone protein localization. (A, B) Tg(centrin:GFP) expression (green) localized to the apical inner segments in wild-type and ahi1lri46 mutants at 3 dpf. Zpr1 (red) was used to stain cone photoreceptors to visualize the apical boundary. (C, D) Five dpf retinas stained with acetylated tubulin (green) and Ift88 (red) showed ciliary localization of the IFT particle in wild-type and mutant animals. (E, H) The transition zone proteins Cep290 and Cc2d2a (red) exhibited punctate staining in wild-type and mutant animals. Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cc2d2a Ab1-cc2d2a WT standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-cc2d2a ahi1lri46/lri46 standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
cep290 WT standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
ahi1lri46/lri46 standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
GFP cu6Tg standard conditions Protruding-mouth eye photoreceptor cell photoreceptor inner segment IHC
cu6Tg ; ahi1lri46 standard conditions Protruding-mouth eye photoreceptor cell photoreceptor inner segment IHC
ift88 Ab1-ift88 WT standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-ift88 ahi1lri46/lri46 standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
IHC ahi1lri46/lri46 standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
zpr-1 IHC cu6Tg standard conditions Protruding-mouth retinal cone cell photoreceptor connecting cilium
IHC cu6Tg ; ahi1lri46 standard conditions Protruding-mouth retinal cone cell photoreceptor connecting cilium
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Day 5 eye photoreceptor cell cilium assembly process quality, normal
Day 5 eye photoreceptor cell photoreceptor connecting cilium cc2d2a expression decreased amount, abnormal
cu6Tg ; ahi1lri46 standard conditions Protruding-mouth eye photoreceptor cell cilium assembly process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.