ZFIN ID: ZDB-FIG-170227-6
Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science   58:448-460 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 6

Immunohistochemical analysis of rod photoreceptors in ahi1lri46 mutants. (A, B) Rod photoreceptors stained with 1D1 (red, rhodopsin) at 5 dpf. (C–F) Rod photoreceptors stained with zpr3 (red, rhodopsin) at 3- (C, D) and 5- (E, F) month-old animals. Arrows in (D) and (F) highlight rhodopsin mislocalization. (G) Quantification of rod outer segment area at 5 dpf. (H) Quantification of rod density at 5 dpf. Scale bars: 20 μm (A–B) and 50 μm (C–F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rho Ab1-rho WT standard conditions Day 5 retinal rod cell photoreceptor outer segment IHC
Ab1-rho ahi1lri46/lri46 standard conditions Day 5 retinal rod cell photoreceptor outer segment IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-3 IHC WT standard conditions Days 45-89 retinal rod cell photoreceptor outer segment
IHC Adult retinal rod cell photoreceptor outer segment
IHC ahi1lri46/lri46 standard conditions Days 45-89 retinal outer nuclear layer
IHC Days 45-89 retinal rod cell photoreceptor outer segment
IHC Adult retinal outer nuclear layer
IHC Adult retinal rod cell photoreceptor outer segment
Phenotype Details
Fish Conditions Stage Phenotype
ahi1lri46/lri46 standard conditions Day 5 retinal rod cell photoreceptor outer segment area, normal
ahi1lri46/lri46 standard conditions Days 45-89 retinal outer nuclear layer zpr-3 labeling mislocalised, abnormal
ahi1lri46/lri46 standard conditions Adult retinal outer nuclear layer zpr-3 labeling mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.