ZFIN ID: ZDB-FIG-161208-4
Topczewska et al., 2016 - The Morphogenesis of Cranial Sutures in Zebrafish. PLoS One   11:e0165775 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 30-44

Fig. S1

RNA detection for genetic markers of osteoblasts in juvenile animals assessed using RNAscope in situ hybridization on paraffin section.

(A-I) Sequential sections (4 μm) of the interfrontal suture collected from juvenile zebrafish at age of 6 wpf. The expression of individual genes is visualized in red, counterstained with haematoxylin for nuclei in purple; black arrows indicate assumed positive expression. The expression of following genes is shown: (A) col2a1a, (B) runx2a, (C) spp1, (D) fgfr1a, (E) fgfr1b, (F) fgfr2, (G) twist2, (H) twist3, (I) foxq1a. The scale bar represents 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
col2a1a AB standard conditions Days 30-44 Not Detected inter-frontal joint osteoblast ISH
fgfr1a AB standard conditions Days 30-44 inter-frontal joint osteoblast ISH
fgfr1b AB standard conditions Days 30-44 inter-frontal joint osteoblast ISH
fgfr2 AB standard conditions Days 30-44 inter-frontal joint osteoblast ISH
foxq1a AB standard conditions Days 30-44 inter-frontal joint ISH
Days 30-44 mesenchymal cell ISH
runx2a AB standard conditions Days 30-44 inter-frontal joint osteoblast ISH
spp1 AB standard conditions Days 30-44 inter-frontal joint osteoblast ISH
twist2 AB standard conditions Days 30-44 Not Detected inter-frontal joint ISH
twist3 AB standard conditions Days 30-44 Not Detected inter-frontal joint ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One