ZFIN ID: ZDB-FIG-161130-5
Grainger et al., 2016 - Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells. Cell Reports   17:1595-1606 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 5

Wnt9a Affects Endothelial gata2b+ G1-S Cell Cycle Progression through myca

(A) Endothelial cells (mCherry+) were collected by FACS from hs:dntcf:IRES-GFP; kdrl:mCherry fish at 30 hpf after heat shock at 16 hpf (n = 100 embryos per condition).

(B) Wnt inhibited (dntcf;GFP+) cells were compared to control (GFP−) cells by qPCR after being sorted by FACS.

(C and D) AB fish were injected with wnt9a MO, myca mRNA, or both; phldb4:Gal4 fish were injected with wnt9a MO, UAS;myca plasmid, and transposase mRNA, fixed at 40 hpf, analyzed by WISH for cmyb (C), and quantified in (D).

Scale bar, 100 μm. p < 0.05; ∗∗∗p < 0.001; n.s., not significant. Error bars represent SD. See also Figure S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 w26Tg ; y171Tg standard conditions Prim-15 endothelial cell RTPCR
ccnd2b w26Tg ; y171Tg standard conditions Prim-15 endothelial cell RTPCR
cdk2 w26Tg ; y171Tg standard conditions Prim-15 endothelial cell RTPCR
cdk4 w26Tg ; y171Tg standard conditions Prim-15 endothelial cell RTPCR
myb AB standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
AB + MO2-wnt9a standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
myca w26Tg ; y171Tg standard conditions Prim-15 endothelial cell RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt9a standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell myb expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
w26Tg ; y171Tg standard conditions Prim-15 endothelial cell cdk2 expression decreased amount, abnormal
w26Tg ; y171Tg standard conditions Prim-15 endothelial cell ccnd1 expression decreased amount, abnormal
w26Tg ; y171Tg standard conditions Prim-15 endothelial cell ccnd2b expression decreased amount, abnormal
w26Tg ; y171Tg standard conditions Prim-15 endothelial cell cdk4 expression decreased amount, abnormal
w26Tg ; y171Tg standard conditions Prim-15 endothelial cell myca expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.