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ZIRC
ZFIN ID: ZDB-FIG-160608-17
Chen et al., 2016 - Cyp2aa9 regulates haematopoietic stem cell development in zebrafish. Scientific Reports   6:26608 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 2

The non-HSC tissues are unaffected in the cyp2aa9 morphants.

(a-d) The cyp2aa9 morphants in bright-field were morphologically normal (a, n = 110/110; b, n = 125/130) and with intact vasculature at 33 hpf under the Tg(kdrl:GFP) background (c, n = 21/21; d, n = 21/25). (e-t) The cyp2aa9 morphants displayed normal expressions of the following tissue-specific markers: primitive haematopoiesis (etsrp, e,f; scl, g,h; gata1, i,j), notochord (shh, k,l), dorsal aorta (flt1, m,n; ephrinB2, o,p) and posterior cardinal vein (flt4, q,r; dab2, s,t). (a-d,i-t) Lateral views, anterior left, dorsal up. (e-h) Dorsal views, anterior left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dab2 WT standard conditions Prim-25 posterior cardinal vein ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 posterior cardinal vein ISH
efnb2a WT standard conditions Prim-5 dorsal aorta ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-5 dorsal aorta ISH
etsrp WT standard conditions 5-9 somites hematopoietic multipotent progenitor cell ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions 5-9 somites hematopoietic multipotent progenitor cell ISH
flt1 WT standard conditions Prim-5 dorsal aorta ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-5 dorsal aorta ISH
flt4 WT standard conditions Prim-5 posterior cardinal vein ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-5 posterior cardinal vein ISH
gata1a WT standard conditions Prim-5 hematopoietic multipotent progenitor cell ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-5 hematopoietic multipotent progenitor cell ISH
shha WT standard conditions Prim-25 notochord ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-25 notochord ISH
tal1 WT standard conditions 14-19 somites hematopoietic multipotent progenitor cell ISH
WT + MO1-cyp2aa3,cyp2aa9 standard conditions 14-19 somites hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-15 whole organism morphology, normal
s843Tg + MO1-cyp2aa3,cyp2aa9 standard conditions Prim-15 vasculature morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.