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ZFIN ID: ZDB-FIG-160215-2
Furukawa et al., 2015 - Induction of Phosphoenolpyruvate Carboxykinase (PEPCK) during Acute Acidosis and Its Role in Acid Secretion by V-ATPase-Expressing Ionocytes. International journal of biological sciences   11(6):712-725 Full text @ Int. J. Biol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Term:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Unillustrated author statements

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
g6pc1a.1 AB standard conditions Protruding-mouth to Day 5 whole organism RTPCR
AB acidic Protruding-mouth to Day 5 whole organism RTPCR
g6pc1a.2 AB standard conditions Protruding-mouth to Day 5 whole organism RTPCR
AB acidic Protruding-mouth to Day 5 whole organism RTPCR
gls2a AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
gls2b AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
glsa AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
glsb AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
glsl AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
glud1a AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
glud1b AB standard conditions Protruding-mouth whole organism RTPCR
AB acidic Protruding-mouth whole organism RTPCR
pck1 Ab1-pck1 AB standard conditions Protruding-mouth whole organism WB
Protruding-mouth to Day 5 whole organism RTPCR
Ab1-pck1 AB acidic Protruding-mouth whole organism WB
Protruding-mouth to Day 5 whole organism RTPCR
Ab1-pck1 AB + MO1-pck1 standard conditions Protruding-mouth Not Detected whole organism WB
Ab1-pck1 AB + MO1-pck1 acidic Protruding-mouth whole organism WB
pck2 AB standard conditions Protruding-mouth to Day 5 whole organism RTPCR
AB acidic Protruding-mouth to Day 5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-atp6v1a WB AB standard conditions Protruding-mouth whole organism
WB AB acidic Protruding-mouth whole organism
WB AB + MO1-pck1 standard conditions Protruding-mouth whole organism
WB AB + MO1-pck1 acidic, chemical treatment: estrogen receptor agonist Protruding-mouth whole organism
WB AB + MO1-pck1 acidic Protruding-mouth whole organism
Phenotype Details
Fish Conditions Stage Phenotype
AB acidic Protruding-mouth glucose homeostasis process quality, abnormal
Protruding-mouth whole organism gls2b expression increased amount, abnormal
Protruding-mouth whole organism glsa expression decreased amount, abnormal
Protruding-mouth whole organism glsl expression increased amount, abnormal
Protruding-mouth whole organism pck1 expression increased amount, abnormal
AB + MO1-pck1 standard conditions Protruding-mouth energy coupled proton transmembrane transport, against electrochemical gradient decreased process quality, abnormal
Protruding-mouth glucose homeostasis process quality, abnormal
Protruding-mouth glutamate homeostasis process quality, normal
Protruding-mouth whole organism pck1 expression absent, abnormal
Protruding-mouth whole organism ab3-atp6v1a labeling decreased amount, abnormal
AB + MO1-pck1 acidic, chemical treatment: estrogen receptor agonist Protruding-mouth energy coupled proton transmembrane transport, against electrochemical gradient process quality, ameliorated
Protruding-mouth vH ionocyte increased amount, abnormal
Protruding-mouth whole organism ab3-atp6v1a labeling decreased amount, abnormal
AB + MO1-pck1 acidic Protruding-mouth energy coupled proton transmembrane transport, against electrochemical gradient decreased process quality, abnormal
Protruding-mouth glucose homeostasis process quality, abnormal
Protruding-mouth glutamate homeostasis process quality, abnormal
Protruding-mouth vH ionocyte increased amount, abnormal
Protruding-mouth whole organism pck1 expression decreased amount, abnormal
Protruding-mouth whole organism ab3-atp6v1a labeling decreased amount, abnormal
Full text @ Int. J. Biol. Sci.