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ZFIN ID: ZDB-FIG-160208-18
Sisson et al., 2015 - A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis. Mechanisms of Development   138 Pt 3:279-90 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-15

Fig. 3

Cranial neural crest cell migration and specification is unaffected in gpc4 and wnt5b mutants. (A–O) Neural crest specification appears normal within gpc4 and wnt5b mutants Scale bar = 100 µm. (A–I) Pre-migratory neural crest markers. 6 somites. (J–O)Pharyngeal arch markers. (J–L) 28 hpf. (M–O) 32 hpf. (P–R) Migration of neural crest appears to be unaffected in gpc4 and wnt5b mutants when compared to wild type fish. Tg(fli1a:EGFP)y1 embryos have neural crest derived cells labeled by GFP expression. Embryos were fixed at 33 hpf and stained with the Alcama (Zn5) antibody (red). Pharyngeal arches are numbered. Scale bar = 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT control Prim-5 pharyngeal arch ISH
gpc4fr6/fr6 control Prim-5 pharyngeal arch ISH
wnt5bta98/ta98 control Prim-5 pharyngeal arch ISH
foxd3 WT standard conditions 5-9 somites premigratory neural crest ISH
gpc4fr6/fr6 standard conditions 5-9 somites premigratory neural crest ISH
wnt5bta98/ta98 standard conditions 5-9 somites premigratory neural crest ISH
hand2 WT control Prim-15 pharyngeal arch ISH
gpc4fr6/fr6 control Prim-15 pharyngeal arch ISH
wnt5bta98/ta98 control Prim-15 pharyngeal arch ISH
pax3a WT standard conditions 5-9 somites premigratory neural crest ISH
gpc4fr6/fr6 standard conditions 5-9 somites premigratory neural crest ISH
wnt5bta98/ta98 standard conditions 5-9 somites premigratory neural crest ISH
sox10 WT standard conditions 5-9 somites premigratory neural crest ISH
gpc4fr6/fr6 standard conditions 5-9 somites premigratory neural crest ISH
wnt5bta98/ta98 standard conditions 5-9 somites premigratory neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 control Prim-5 neural crest cell fate specification process quality, normal
Prim-15 neural crest cell fate specification process quality, normal
gpc4fr6/fr6 standard conditions 5-9 somites convergent extension process quality, abnormal
5-9 somites neural crest cell fate specification process quality, normal
wnt5bta98/ta98 control Prim-5 neural crest cell fate specification process quality, normal
Prim-15 neural crest cell fate specification process quality, normal
wnt5bta98/ta98 standard conditions 5-9 somites convergent extension process quality, abnormal
5-9 somites neural crest cell fate specification process quality, normal
y1Tg ; gpc4fr6/fr6 control Prim-15 convergent extension process quality, abnormal
Prim-15 neural crest cell migration process quality, normal
Prim-15 whole organism anterior-posterior axis decreased length, abnormal
y1Tg ; wnt5bta98/ta98 control Prim-15 convergent extension process quality, abnormal
Prim-15 neural crest cell migration process quality, normal
Prim-15 whole organism anterior-posterior axis decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Sisson, B.E., Dale, R.M., Mui, S.R., Topczewska, J.M., Topczewski, J., A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis, 279-90, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.