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ZFIN ID: ZDB-FIG-160208-19
Sisson et al., 2015 - A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis. Mechanisms of Development   138 Pt 3:279-90 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 4

The gpc4 mutant craniofacial phenotype is not enhanced by the loss of wnt5b. (A–B) The length of the symplectic element (see inset) from 5 dpf embryos. (A) Measurements of the symplectics from progeny of a dihybrid cross of gpc4m818 and wnt5bta98. The presence of wnt5bta98 does not enhance the gpc4m818 phenotype, but the double heterozygous mutant is significantly different than wild type. Tests of 14 a priori hypotheses were conducted using a Bonferroni adjusted alpha level of 0.00357 (0.05/11). *p = 0.0015, Mann–Whitney U (df) = 20. **p = 0.0021, Mann–Whiney U (df) = 88. (B) The symplectic cartilage is significantly smaller in gpc4fr6 mutants than the symplectic elements in gpc4m818 mutants. *p = 0.0157, Mann–Whitney U (df) = 22. Abbreviations: sy = symplectic, hm = hyomandibular.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 standard conditions Day 5 symplectic decreased length, abnormal
gpc4m818/+ standard conditions Day 5 symplectic length, normal
gpc4m818/+ ; wnt5bta98/+ standard conditions Day 5 symplectic decreased length, abnormal
gpc4m818/+ ; wnt5bta98/ta98 standard conditions Day 5 symplectic decreased length, abnormal
gpc4m818/m818 standard conditions Day 5 symplectic decreased length, abnormal
gpc4m818/m818 ; wnt5bta98/+ standard conditions Day 5 symplectic decreased length, abnormal
wnt5bta98/+ standard conditions Day 5 symplectic length, normal
wnt5bta98/ta98 standard conditions Day 5 symplectic decreased length, abnormal
wnt5bta98/ta98 ; gpc4m818/m818 standard conditions Day 5 symplectic decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Sisson, B.E., Dale, R.M., Mui, S.R., Topczewska, J.M., Topczewski, J., A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis, 279-90, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.