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ZFIN ID: ZDB-FIG-160208-20
Sisson et al., 2015 - A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis. Mechanisms of Development   138 Pt 3:279-90 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 5

Craniofacal cartilage volume is reduced in gpc4 mutants while the numbers of cells is unchanged. (A–C) Wheat germ agglutinin (gray) and DAPI (blue) staining of ceratohyal elements. (D) A 3D reconstruction of 20 cells in the center of a wild type ceratohyal stained with WGA to determine cell volume. (E) There is a significant difference between the average length of the ceratohyal and symplectic elements between the wild type and mutants. All are p = < 0.001. (F) The average number of cells in ceratohyal and symplectic elements. (G) The average volume of the ceratohyal and symplectic elements. The ratio of the ceratohyal element volume is Vfr6/VWT = 0.63. (E–G) Tests of four a priori hypotheses were conducted using a Bonferroni adjusted alpha level of 0.0125 (0.05/4). *p < 0.0125. (H) The average volume of 20 cells in the center and proximal ends from three ceratohyal elements from gpc4fr6 embryos and their phenotypically wild type siblings. Tests of two a priori hypotheses were conducted using a Bonferroni adjusted alpha level of 0.025 (0.05/2). *p < 0.025. The ratio of the center chondrocyte volume is Vfr6/VWT = 0.71. The ratio of the proximal chondrocyte volume is Vfr6/VWT = 0.74. Scale bar = 20 µm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 standard conditions Day 4 ceratohyal cartilage decreased length, abnormal
Day 4 ceratohyal cartilage decreased volume, abnormal
Day 4 ceratohyal cartilage chondrocyte amount, normal
Day 4 ceratohyal cartilage chondrocyte decreased size, abnormal
Day 4 symplectic decreased length, abnormal
Day 4 symplectic decreased volume, abnormal
wnt5bta98/ta98 standard conditions Day 4 ceratohyal cartilage decreased length, abnormal
Day 4 ceratohyal cartilage chondrocyte amount, normal
Day 4 symplectic decreased length, abnormal
Day 4 symplectic cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Sisson, B.E., Dale, R.M., Mui, S.R., Topczewska, J.M., Topczewski, J., A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis, 279-90, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.