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ZFIN ID: ZDB-FIG-160208-17
Sisson et al., 2015 - A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis. Mechanisms of Development   138 Pt 3:279-90 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2

A subset of Wnt/PCP molecules influence craniofacial cartilage development. (A–F) Not all identified core Wnt/PCP zebrafish mutants have a craniofacial chondrocyte stacking defect. Confocal images of whole-mount Wheat Germ Agglutanin (WGA) staining (gray) of Wnt/PCP mutant embryo heads at 4 dpf. Scale bar = 50 µm. (G–L) Confocal images of a single focal plan of WGA staining (gray) of Wnt/PCP mutant craniofacial cartilage elements at 4 dpf. Nuclei were labeled with DAPI (blue). Scale bar = 20 µm. M. Graph of length to width ratio. The y-axis is all 40 chondrocyte measurements from 4 different ceratohyals for wild type and mutant genotypes. The x-axis is the length to width ratio with a graphical representation of select ratios. The mean LWR and their respective standard deviation is marked by red cross-hairs and stated in Supplemental Table 1. To determine if data could be compared pairwise the D′Agostino and Pearson omnibus normality test was used. Mann–Whitney U was determined and the statistical significances between the means is marked on graph and stated in Supplemental Table 1. Abbreviations: M = Meckel′s cartilage, CH = ceratohyal, CB = ceratobranchial, HM = hyomandibular, IH = interhyal joint, and PQ = palatoquadrate. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpc4fr6/fr6 standard conditions Day 4 ceratohyal cartilage condensed, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 ceratohyal cartilage perpendicular to whole organism anterior-posterior axis, abnormal
Day 4 ceratohyal cartilage chondrocyte circular, abnormal
Day 4 Meckel's cartilage condensed, abnormal
Day 4 Meckel's cartilage deformed, abnormal
Day 4 Meckel's cartilage perpendicular to whole organism anterior-posterior axis, abnormal
Day 4 Meckel's cartilage shape, abnormal
Day 4 Meckel's cartilage chondrocyte circular, abnormal
Day 4 pharyngeal arch cartilage deformed, abnormal
scribrw468/rw468 standard conditions Day 4 ceratohyal cartilage bent, abnormal
Day 4 ceratohyal cartilage deformed, abnormal
Day 4 Meckel's cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage chondrocyte stacked, normal
vangl2m209/m209 standard conditions Day 4 ceratohyal cartilage deformed, abnormal
Day 4 Meckel's cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage chondrocyte stacked, normal
wnt11tz216/tz216 standard conditions Day 4 ceratohyal cartilage deformed, abnormal
Day 4 Meckel's cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage cartilage element mislocalised, abnormal
Day 4 pharyngeal arch cartilage chondrocyte stacked, normal
wnt5bta98/ta98 standard conditions Day 4 ceratohyal cartilage deformed, abnormal
Day 4 Meckel's cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage deformed, abnormal
Day 4 pharyngeal arch cartilage cartilage element decreased length, abnormal
Day 4 pharyngeal arch cartilage chondrocyte circular, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Sisson, B.E., Dale, R.M., Mui, S.R., Topczewska, J.M., Topczewski, J., A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis, 279-90, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.