ZFIN ID: ZDB-FIG-151214-53
Takeuchi et al., 2015 - LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2. Proceedings of the National Academy of Sciences of the United States of America   112(45):13922-7 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 3

Up-regulation of endothelial markers in lsd1it627 embryos. (A) Expression of etv2 at the indicated developmental stages in WT or lsd1it627 embryos. The arrowhead indicates the up-regulation of etv2 in the posterior LPM. (B) Expression of fli1a at 21 hpf in WT or lsd1it627 embryos. The arrowhead indicates the up-regulation of fli1a in the ICM. (C) Expression of fli1a, flk1, tie1, and biklf at 26 hpf in the trunk region. The blue and red arrows indicate up-regulation of endothelial markers and down-regulation of a hematopoietic marker, respectively, in the hematopoietic region. (Right) Diagram of the hematopoietic and endothelial regions in WT embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etsrp AB/TL standard conditions 1-4 somites lateral plate mesoderm ISH
5-9 somites lateral plate mesoderm ISH
kdm1ait627/it627(AB/TL) standard conditions 1-4 somites lateral plate mesoderm ISH
5-9 somites anterior lateral plate mesoderm ISH
5-9 somites lateral plate mesoderm ISH
5-9 somites posterior lateral plate mesoderm ISH
fli1 AB/TL standard conditions 20-25 somites angioblastic mesenchymal cell ISH
Prim-5 endothelial cell ISH
kdm1ait627/it627(AB/TL) standard conditions 20-25 somites angioblastic mesenchymal cell ISH
Prim-5 endothelial cell ISH
kdrl AB/TL standard conditions Prim-5 endothelial cell ISH
kdm1ait627/it627(AB/TL) standard conditions Prim-5 endothelial cell ISH
klf17 AB/TL standard conditions Prim-5 hematopoietic system ISH
kdm1ait627/it627(AB/TL) standard conditions Prim-5 hematopoietic system ISH
tie1 AB/TL standard conditions Prim-5 endothelial cell ISH
kdm1ait627/it627(AB/TL) standard conditions Prim-5 endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kdm1ait627/it627(AB/TL) standard conditions 5-9 somites posterior lateral plate mesoderm etsrp expression increased amount, abnormal
20-25 somites angioblastic mesenchymal cell fli1 expression increased amount, abnormal
Prim-5 endothelial cell tie1 expression increased amount, abnormal
Prim-5 endothelial cell kdrl expression increased amount, abnormal
Prim-5 endothelial cell fli1 expression increased amount, abnormal
Prim-5 hematopoietic system klf17 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA