Fig. 4

Rescue of the hematopoietic defects in lsd1^it627 embryos by etv2 knockdown. (A) Expression of gata1 in WT or lsd1^it627 embryos injected or not injected with 0.5 pmol of the indicated morpholino oligonucleotides. The down-regulation of gata1 in lsd1^it627 embryos (open arrowheads) was rescued by the etv2MO injection (closed arrowheads). 5mis, 5-mismatch. (B) Expression of pu.1 at 24 hpf in WT or lsd1^it627 embryos injected or not injected with 0.5 pmol of the indicated morpholino oligonucleotides. The gata1MO-induced ectopic pu.1 expression in the ICM (arrows) was lost in lsd1^it627 embryos (open arrowhead) and recovered by the coinjection of etv2MO (closed arrowhead). (C) Methylation status of histone H3-K4 on the etv2 gene in 15-hpf embryos injected (gray bars) or not injected (white bars) with 1 pmol of lsd1MO (lsd1MO inj). Genomic regions (up1, int2, 35bp, ex8) examined by ChIP are shown as black bars. The results are means ± SEs of three independent experiments. *P < 0.05; Student’s t test.>