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ZFIN ID: ZDB-FIG-150824-12
Helker et al., 2015 - The hormonal peptide Elabela guides angioblasts to the midline during vasculogenesis. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to 20-25 somites

Fig. 2, S1 Expression of aplnra, aplnrb, apln and ela coincides with the formation of the DA and PCV.

(AD) In situ hybridizations detecting expression of aplnra (A), aplnrb (B), apln (C) and ela (D) at the indicated time points (12 hpf, 18 hpf shown in dorsal views, 20 hpf in lateral views). aplnrs are expressed in the migrating angioblasts and continue to be expressed during formation of the DA and PCV. At 12 hpf, aplnrb is strongly expressed in the angioblasts (prior to migration, arrowheads) and within the lateral plate mesoderm and at the notochord somite boundary. aplnra expression is strong in the paraxial mesoderm (A). Both receptors are expressed in the angioblasts at 18 hpf (A, B, arrowheads) and in the developing axial vessels at 20 hpf (Arrowheads point to the DA, arrows to the PCV). Prior to angioblast migration apln and ela are both expressed by the notochord, with ela showing a much stronger expression (C, D). After angioblasts reached the midline (18 hpf, 20 hpf) only apln is still expressed by the notochord, whereas notochord derived ela expression is strongly reduced to absent. (E) Knockdown of aplnr does not influence the specification of angioblasts. In situ hybridizations detecting expression of the angioblast marker etv2 at 13.5 hpf (dorsal views).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apela WT standard conditions 5-9 somites notochord ISH
14-19 somites notochord ISH
20-25 somites notochord ISH
apln WT standard conditions 5-9 somites notochord ISH
14-19 somites Not Detected notochord ISH
20-25 somites Not Detected notochord ISH
aplnra WT standard conditions 5-9 somites paraxial mesoderm ISH
14-19 somites angioblastic mesenchymal cell ISH
14-19 somites blood vessel endothelial cell ISH
20-25 somites vascular cord ISH
aplnrb WT standard conditions 5-9 somites angioblastic mesenchymal cell ISH
5-9 somites blood vessel endothelial cell ISH
5-9 somites lateral plate mesoderm ISH
14-19 somites angioblastic mesenchymal cell ISH
14-19 somites blood vessel endothelial cell ISH
20-25 somites vascular cord ISH
etsrp WT control 5-9 somites angioblastic mesenchymal cell ISH
5-9 somites blood vessel endothelial cell ISH
WT + MO1-aplnra + MO1-aplnrb control 5-9 somites angioblastic mesenchymal cell ISH
5-9 somites blood vessel endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife