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ZFIN ID: ZDB-FIG-150824-14
Helker et al., 2015 - The hormonal peptide Elabela guides angioblasts to the midline during vasculogenesis. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 14-19 somites

Fig. 3 Notochord (NC) ela expression is sufficient to guide angioblasts to the midline.

(A) Angioblasts fail to migrate to the midline position in the NC mutants noto and ta. Confocal projections of Tg(fli1a:EGFP)y1 embryos in dorsal view at 17 hpf (scale bars: 20 µm). (B) In situ hybridization in 17 hpf old zebrafish embryos showing ela expression from the NC and somites (white arrows). NC deficiency abolishes or strongly reduces ela expression in noto or ta mutant embryos. (C) Mosaic expression of Ela in the midline, achieved by injection of shh:ela DNA, led to rescue of angioblast migration in tab160 mutant embryos (n = 61 embryos, ctr injected n = 32 embryos). Significance was calculated by chi-square (Χ2 = 19.65) equivalent to p < 0.001 (p = 9E-06). Scale bars: 30 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apela nototk241/tk241; y1Tg control 14-19 somites notochord ISH
14-19 somites notochord anatomical region ISH
tbxtab160/b160; y1Tg control 14-19 somites notochord ISH
14-19 somites notochord anatomical region ISH
y1Tg control 14-19 somites notochord ISH
14-19 somites notochord anatomical region ISH
EGFP nototk241/tk241; y1Tg control 14-19 somites angioblastic mesenchymal cell IFL
14-19 somites blood vessel endothelial cell IFL
tbxtab160/b160; y1Tg control 14-19 somites angioblastic mesenchymal cell IFL
14-19 somites blood vessel endothelial cell IFL
y1Tg control 14-19 somites vascular cord IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nototk241/tk241; y1Tg control 14-19 somites angioblast cell migration from lateral mesoderm to midline arrested, abnormal
14-19 somites angioblast cell migration from lateral mesoderm to midline process quality, abnormal
tbxtab160/b160; y1Tg control 14-19 somites angioblast cell migration from lateral mesoderm to midline arrested, abnormal
14-19 somites angioblast cell migration from lateral mesoderm to midline process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife