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ZIRC
ZFIN ID: ZDB-FIG-140325-13
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

Zebrafish pericytes express notch3. (A,B) notch3 expression in the trunk vessels. Arrowheads, DA; arrow, ISVs; asterisk, neural expression. (C,D) Fluorescence ISH showing notch3 expression in trunk (C) and brain pericytes (D). Arrowheads indicate notch3+ pericytes and asterisk marks neural expression. Inset shows a high magnification view of a notch3+ pericyte. (E) Double fluorescence ISH showing colocalization of pdgfrb and notch3 transcripts in brain pericytes. Asterisks mark neural expression of notch3. (F,G) Double fluorescence ISH showing colocalization of pdgfrb and notch3 transcripts in trunk vessels. (F) Confocal section showing ISV pericytes. (G) DA mural cells. Arrows indicate pdgfrb+ notch3+ pericytes. Insets show high magnification views of pdgfrb and notch3 co-expression in pericytes. CtA, central artery; DA, dorsal aorta; ISV, intersegmental vessels. Scale bars: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Protruding-mouth central artery IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
notch3 WT standard conditions Long-pec dorsal aorta pericyte ISH
Long-pec spinal cord ISH
Protruding-mouth dorsal aorta pericyte ISH
Protruding-mouth intersegmental vessel pericyte ISH
y1Tg standard conditions Protruding-mouth central artery pericyte ISH
Protruding-mouth dorsal aorta pericyte ISH
Protruding-mouth intersegmental vessel pericyte ISH
Protruding-mouth spinal cord ISH
pdgfrb y1Tg standard conditions Protruding-mouth central artery pericyte ISH
Protruding-mouth dorsal aorta pericyte ISH
Protruding-mouth intersegmental vessel pericyte ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development